| RESEARCH ARTICLE |
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| Year : 2015 | Volume
: 4
| Issue : 1 | Page : 65-74 |
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The effect of cis and trans vaccenic acids on expression of ICAM-1 and VCAM-1 in human microvascular endothelial cells (HMEC)
Abbas Abbasi1, Ali Mostafaie1, Gholamreza Bahrami1, Kamran Mansouri2, Sajad Sisakhtnejad1
1 Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran
2 Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah; Department of Molecular Medicine, School of advanced Medical Technologies, Tehran University of Medical Sciences, Tehran, Iran
Correspondence Address:
Ali Mostafaie
Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah
Iran
Kamran Mansouri
Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah; Department of Molecular Medicine, School of advanced Medical Technologies, Tehran University of Medical Sciences, Tehran
Iran
 Source of Support: None, Conflict of Interest: None  | Check |
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Trans-fatty acids (TFA) are known as a risk factor for coronary artery diseases. Trans-11 vaccenic acid [VA; 18:1(n-9)], known as the major trans fatty acid in milk fat, is a positional and geometric isomer of oleic acid and a precursor of conjugated linoleic acid (CLA) in human . This study was carried out in order to investigate the effect of Cis and Trans Vaccenic Acids on expression of intra cellular adhesion molecul-1 (ICAM-1) and vascular cellular adhesion molecul-1 (VCAM-1) in human Microvascular endothelial cells (HMECs). To induce adhesion molecules expression, TNF-α or LPS were added to HMECs in a cell culture being treated with Cis and Trans vaccenic acid. In the next step, to evaluate ICAM-1 and VCAM-1 expression before and after the treatment, Western blot technique was used. The analyzed results indicated that Cis and Trans vaccenic acids are able to suppress VCAM-1 and ICAM-1 expression on HMEC near to the basal level. Also it has been found that cis vaccenic acid isomer could suppress both VCAM-1 and ICAM-1 expression as well as trans isomer. Together all and considering the role of mentioned adhesion molecules in atherosclerosis; it seems that Cis and trans vaccenic acids could be a possible prophylaxis agents in populations with high risk of atherosclerosis.
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