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RESEARCH ARTICLE
Year : 2016  |  Volume : 5  |  Issue : 2  |  Page : 104-111

Study of callus induction and cell culture to secondary metabolite production in Hyssopus officinalis L.


1 Department of Medicinal Plants, Institute of Higher Education, Jahad-e-Daneshgahi, Kermanshah Unit, Kermanshah, Iran
2 Agronomy and Plant Breeding Department, Razi University; Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran
3 Medical Biology Research Center, Kermanshah University of Medical Sciences; Zagros Bioidea Co. Razi University Incubator, Kermanshah, Iran
4 Medical Biology Research Center, Kermanshah University of Medical Sciences; Department of Medicinal Plants, Institute of Higher Education, Jahad-e-Daneshgahi, Kermanshah Unit, Kermanshah, Iran

Correspondence Address:
Danial Kahrizi
Agronomy and Plant Breeding Department, Razi University; Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah
Iran
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Source of Support: None, Conflict of Interest: None


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The Hyssopus officinalis L. is an important medicinal plant. Antimicrobial and antifungal activities of the essential oil of hyssop have been reported. In this article the effects of explants types, plants growth regulators and elicitors on callus induction and cell culture conditions were studied. In this experiment it was found that there were non- significant differences among hypocotyl and leaf H. officinalis explants for callus induction and callus growth rate. There were significant differences among levels of growth regulators and interaction effect between growth regulators and expellant types for callus induction and callus growth rate. There were significant differences among plant growth regulators levels for callus induction and callus growth rate. Results showed that medium supplemented by N2B1 and N0.5B1 showed the highest callus induction and callus growth rate respectively. In this paper it was demonstrated the important role of plant growth regulators and explant types on callus induction in H. Officinalis as a medicinal plants. Also the most important secondary metabolites in H. officinalis were increased in cell culture presence of salicylic acid, citric acid and yeast extract elicitors.


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