• Users Online: 168
  • Home
  • Print this page
  • Email this page
Home About us Editorial board Ahead of print Current issue Search Archives Submit article Instructions Subscribe Contacts Login 
RESEARCH ARTICLE
Year : 2016  |  Volume : 5  |  Issue : 2  |  Page : 61-66

The effect of shallot (Allium Hirtifolium) extract on profile of Th1/Th2 cytokines in Balb/C mouse splenic lymphocytes


1 Department of Immunology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran
2 Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran
3 Department of Immunology, School of Medicine, Kermanshah University of Medical Sciences; Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran

Correspondence Address:
Ali Mostafaie
Department of Immunology, School of Medicine, Kermanshah University of Medical Sciences; Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah
Iran
Login to access the Email id

Source of Support: None, Conflict of Interest: None


Rights and PermissionsRights and Permissions

Modulation of cytokine secretion by phytoconstituents offers a novel approach for treatment of diseases. A class of herbal medicines, known as immunomodulators, alters the activity of immune system through the dynamic regulation of cytokines secretion. Cytokines modulate functions of immune cells, including Th1 (T helper 1) or Th2 responses, responsible for cell-mediated and humoral immunity, respectively. Here, we evaluated the immunomodulatory effects of hydroalcoholic extract of shallot (A. hirtifolium) on immune response in BALB/c mouse. Extract of shallot bulbs were prepared and two doses of extract (80 and 400 mg/kg) were injected intraperitoneally within 10 days. Thereafter, splenic mononuclear cells (SMNC) were isolated and secreted IFN-γ (Interferon-gamma), Il-4 (Interleukin-4) as two prototypes of Th1/Th2 cytokines, and TNF-α (Tumor Necrosis Factor-alpha) levels were assayed using Enzyme-linked Immunosorbent assay (ELISA) kits. The results showed that shallot extract significantly reduced Th2 and Th1 responses by down-regulation of Il-4 and IFN-γ levels. The extract also reduced TNF-α, the major proinflammatory cytokine. These data indicated the suppressive potential of shallot extract on the signature cytokines of Th1/Th2 subsets and TNF-α.


[PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed254    
    Printed31    
    Emailed0    
    PDF Downloaded56    
    Comments [Add]    

Recommend this journal