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Year : 2016  |  Volume : 5  |  Issue : 2  |  Page : 80-82

DNA binding study of dihydropyrano [3, 4-C] chromene derivative by some spectroscopic techniques

Department of Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran

Correspondence Address:
Gholamreza Dehghan
Department of Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz
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Source of Support: None, Conflict of Interest: None

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This study was designed to examine the interaction of dihydropyranochromene derivative, 2-amino-4-phenyl-5-oxo-4H, 5H-pyrano-[3, 2-c] chromene-3-carbonitrile (4-PC) with calf thymus DNA (ctDNA) by absorption spectroscopy, competitive fluorescence studies, circular dichroism (CD) and viscosity measurements. It was found that 4-PC could interact with ctDNA through non- intercalative mode and the intrinsic binding constant, Kb, for 4-PC with ctDNA was estimated to be 2.74 ×103 M-1. Methylene blue (MB) displacement studies revealed that 4-PC could not replace ctDNA bounded MB, which is indicative of groove binding mode of 4-PC with DNA. Furthermore, 4-PC induced detectable changes in the CD spectrum of ctDNA as well as changes in its viscosity, corroborate the above experimental results.

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