• Users Online: 360
  • Home
  • Print this page
  • Email this page
Home About us Editorial board Ahead of print Current issue Search Archives Submit article Instructions Subscribe Contacts Login 
RESEARCH ARTICLE
Year : 2018  |  Volume : 7  |  Issue : 1  |  Page : 79-87

Salt-Assisted Liquid-Liquid Extraction followed by High Performance Liquid Chromatography for Determination of Carvedilol in Human Plasma


Department of Chemistry, Lorestan University, Khorramabad 6813717133, Iran

Correspondence Address:
Login to access the Email id

Source of Support: None, Conflict of Interest: None


Rights and PermissionsRights and Permissions

A fast and simple method for extraction of carvedilol in human plasma samples based on salting out assisted liquid-liquid extraction (SALLE) is described. The method involving extraction of carvedilol with water-miscible organic solvent acetonitrile when solvent phase separation occurs using NaCl as a salt. The extracted phase was analyzed by high- performance liquid chromatography with ultra violet detection at 240 nm. The procedure has been optimized with respect to type and amount of salt, volume of sample, extraction solvent and the pH of solution. In the optimal condition the linear calibration range was 5-500 μg L-1 and the correlation coefficient was 0.9965. The limit of detection and limit of quantification were 1.0 μg L-1 and 3.3 μg L-1, respectively and a relative standard deviation of 3.5 % for five replicates were obtained. In spiking experiments on real samples, the average recoveries found by the present method were between 96.0% and 105.0%.


[PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed125    
    Printed26    
    Emailed0    
    PDF Downloaded45    
    Comments [Add]    

Recommend this journal