• Users Online: 734
  • Home
  • Print this page
  • Email this page
Home About us Editorial board Ahead of print Current issue Search Archives Submit article Instructions Subscribe Contacts Login 
RESEARCH ARTICLE
Year : 2018  |  Volume : 7  |  Issue : 1  |  Page : 9-19

Two phase solvent extraction method for analysis of methadon in immature human milk, a breast feeding recommendation in early postpartum period


1 Department of Obstetrics and Gynecology, Maternity Research Center, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran
2 Department of Medicinal Chemistry, School of Pharmacy-International Campus, University of Medical Sciences, Tehran; Nano Drug Delivery Research Center, Kermanshah University of Medical Science, Kermanshah, Iran

Correspondence Address:
Farhad Ahmadi
Department of Medicinal Chemistry, School of Pharmacy-International Campus, Iran University of Medical Sciences, Tehran; Nano Drug Delivery Research Center, Kermanshah University of Medical Science, Kermanshah
Iran
Login to access the Email id

Source of Support: None, Conflict of Interest: None


Rights and PermissionsRights and Permissions

In this work we introduced a new two phase freezing (TPF) method coupled with gas chromatography for the extraction, clean up and determination of methadone (MT) in human milk samples. TPF procedure was optimized for extraction of MT from immature milk sample. The extraction of MT was performed from 1.0 ml of milk that contain 0.2 ml of Briton Robinson buffer (pH=2.5) and 0.3 ml of acetonitrile. For separation of acetonitrile from aqueous solution, the solution was placed in refrigerator at −40 °C. The MT was analyzed by gas chromatography. The results demonstrated that the amount of MT that transferred to milk is significantly different from other published reports. The immature milks of six women who were used MT (dose of 90 mg/day) in duration of 1, 2, 3, and 5 h after consumption were analyzed. Our data demonstrated that, before one hour and also 5 h after MT consumption the breastfeeding is safe and between 2-4 h after consumption dose not safe neither.


[PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed64    
    Printed6    
    Emailed0    
    PDF Downloaded26    
    Comments [Add]    

Recommend this journal