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ORIGINAL ARTICLE
Year : 2019  |  Volume : 8  |  Issue : 1  |  Page : 68-77

Green peas protein hydrolyzed by bromelain in simple procedure to improve kidney function in cisplatin-induced rats


1 Nutrition Department, Faculty of Medicine, Universitas Kristen Maranatha, Bandung, Indonesia
2 Biochemistry Department, Faculty of Medicine, Universitas Kristen Maranatha, Bandung, Indonesia
3 Biology Department, Faculty of Medicine, Universitas Kristen Maranatha, Bandung, Indonesia
4 Pharmacology Department, Pharmacy School, Instittut Teknologi Bandung, Bandung, Indonesia
5 Biochemistry Department, Jenderal Achmad Yani University, Jawa Barat, Indonesia

Correspondence Address:
Meilinah Hidayat
Faculty of Medicine, Universitas Kristen Maranatha, Jl. Prof. Drg. Suria Sumantri MPH No. 65 Bandung 40164
Indonesia
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/jrptps.jrptps_15_17

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Context: Chronic kidney disease (CKD) can lead to terminal kidney failure. Previous study has shown that protein hydrolysate in yellow peas (Pisum sativum L.) can be used as a natural remedy for CKD. Aims: To obtain hydrolysate protein that is most effective in improving kidney function of cisplatin (CP)-induced Wistar rats, based on urea, creatinine, atrial natriuretic peptide (ANP), cyclooxygenase-1 (COX-1), and renin levels of CP-induced nephrotoxicity Wistar rats. Materials and Methods: Methods of Kjeldahl, Bradford, Kunitz, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis were used to determine the content of the eight types of protein hydrolysates. In in vivo experiment, the samples were administered to CP-induced nephrotoxicity Wistar rats, with urea, creatinine, ANP, COX-1, and renin as parameters. Results: Total neutrase activity was 40.65 U/mg, and bromelain was 35.77 U/mg. Total specific activities of both enzymes were almost identical. Protein hydrolyzed using bromelain had small fractions (<14.4 kDa). On the 30th day of treatment, urea and creatinine levels of all groups of treatment were significantly different from CP control (P < 0.01). The lowest level was shown by the group which was treated with bromelain-hydrolyzed green pea protein. Among ANP, COX-1, and renin measurements, only the result of COX-1 showed the promising result. Conclusions: Green peas protein hydrolysate hydrolyzed by bromelain are suggested as the most effective in improving kidney function based on urea, creatinine, and COX-1 levels of CP-induced nephrotoxicity Wistar rats.


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