|Year : 2019 | Volume
| Issue : 1 | Page : 91-101
New insights into the therapeutic effects of phenolic acids from sorghum seeds
Reda Ben Mrid1, Youssef Bouargalne1, Redouane El Omari2, Mohamed Nhiri1
1 Departement of Biology, Laboratory of Biochemistry and Molecular Genetics, Faculty of Sciences and Technologies of Tangier, Tangier, Morocco
2 Departement of Biology, Laboratory of Biochemistry and Molecular Genetics, Faculty of Sciences and Technologies of Tangier, Tangier; Higher School of Technology (EST) Sidi Bennour, Chouaib Doukkali University, El Jadida, Morocco
|Date of Web Publication||28-Mar-2019|
Department of Biology, Laboratory of Biochemistry and Molecular Genetics, Faculty of Science and Technology, Abdelmalek Essaadi University, Tangier principal BP 416
Source of Support: None, Conflict of Interest: None
This paper reviewed the beneficial effects of the major phenolic acid compounds of Sorghum bicolor seeds. Different studies were reviewed to determine the major phenolic acid components of sorghum seeds. Several kinds of literature were then analyzed to discuss the different beneficial effects of these molecules. S. bicolor is an important source for food and feed. It is among the top five crops regarding its production and consumption throughout the world. Till date, many studies highlighted different aspects of the biochemical and physiological properties of sorghum grain. However, studies concerning the pharmacological properties of sorghum grain are scarce. The predominant phenolic acids of sorghum seeds are ferulic, p-coumaric, and protocatechuic acids. The bioactive effects of these phenolic acids are mainly related to their antioxidant, antitumor, antidiabetic, antimicrobial, cardiovascular, and gastrointestinal activities. The data collected from recent studies indicate that these molecules have a promising future as natural agents for the treatment of various diseases, and this is particularly due to their strong antioxidant properties. This review provides evidence for the importance of sorghum seeds and their phenolic compounds in the prevention and treatment of several diseases. This work showed that sorghum grains are a good source of beneficial and therapeutic molecules. It also recommended the addition of sorghum grains to human diet as other cereals because of its high nutritional value.
Keywords: Ferulic acid, p-coumaric acid, pharmacological effects, phenolic compound, protocatechuic acid, sorghum grain
|How to cite this article:|
Ben Mrid R, Bouargalne Y, El Omari R, Nhiri M. New insights into the therapeutic effects of phenolic acids from sorghum seeds. J Rep Pharma Sci 2019;8:91-101
|How to cite this URL:|
Ben Mrid R, Bouargalne Y, El Omari R, Nhiri M. New insights into the therapeutic effects of phenolic acids from sorghum seeds. J Rep Pharma Sci [serial online] 2019 [cited 2019 Aug 23];8:91-101. Available from: http://www.jrpsjournal.com/text.asp?2019/8/1/91/255056
| Introduction|| |
According to the United Nations Food and Agriculture Organization, sorghum (Sorghum bicolor L. Moench) is the 5th most important cereal in the world after rice, wheat, corn, and barley., In 2015, the world sorghum production was estimated at 66 million tones. This drought-resistant crop is of great nutritional interest, particularly in dry regions, where food security is most at risk and where this plant is one of the main foodstuffs. In these hot regions, sorghum is grown for both its grain for human food and straw for livestock feed. Like all cereals, sorghum grains are mainly composed of starch, protein, nonstarch polysaccharides, and fatty acids. However, sorghum grains do not contain gluten and constitute therefore an important cereal for people intolerant to gluten. Furthermore, sorghum grains are of the same nutritional quality as maize grains [Table 1].,, For all these important characteristics, sorghum would be a grain of the future and could replace or supplement other cereals in the human diet.
Unlike primary metabolites (amino acids, lipids, sugars, and nucleotides), which are directly implicated in plant growth and development, secondary metabolites are molecules indirectly essential to the life of plants. In fact, secondary metabolites may act as structural elements or as important tools in the adaptation of plants to their environment., They thus participate in the tolerance of plants to various stresses as follows: pathogenic attacks of bacteria and fungi, predation of insects, drought, and ultraviolet (UV) light.,, Secondary metabolites can be divided into three major chemical groups in plants: nitrogen compounds, terpenes, and phenolic compounds.
Nitrogen-containing secondary metabolites are molecules with a basic character and are characterized by the presence of nitrogen within their structures. The most common nitrogen compounds in plants are alkaloids, glycosides, and nonprotein amino acids., These metabolites play an important role in plant defense against mammals and insects. For humans, most alkaloids are very toxic; however, these molecules may have a therapeutic effect at low doses. In fact, from prehistory to the present day, alkaloids or alkaloids-containing extracts have been used as muscle relaxants, analgesics, and tranquilizers.
Terpenes are organic molecules derived from 5 carbon isoprene units as a building block. Terpenes can be subdivided in monoterpenes (C10), sesquiterpenes (C15), diterpenes (C20), triterpenes (C30), tetraterpenes (C40), and the polyterpenes with more than 40 carbons., Volatile monoterpenes and sesquiterpenes are the main components of essential oils., The most common terpenes are triterpenes, tetraterpenes, and polyterpenes. These metabolites serve as anti-herbivore defense compounds in plants; however, some terpenes are also important for plant development such as gibberellins, carotenoids, and brassinosteroids.,,
Phenolic compounds are aromatic molecules consisting of a phenyl (C6) group attached to a hydroxyl (−OH) group. The structure of these molecules varies from simple molecules (simple phenolic acids) to highly polymerized molecules (condensed tannins). Phenolic compounds can be classified into five subgroups: lignins, flavonoids, tannins, phenolic acids, and coumarins. In addition to their implication in plant structure development; phenolic compounds are associated with several physiological processes such as defense against pathogens, insects, and other animals., Phenolic compounds may also be attractive to insects and other animals and act as pollinators. These compounds are also known for their antioxidant, anti-inflammatory, antiatherogenic, antithrombotic, analgesic, antibacterial, antiviral, anticancer, cardiovascular, and gastrointestinal activities.,,,,
| Search Method|| |
A search was carried out to identify appropriate published articles on electronic databases including Science Direct, PubMed, and Google Scholar. The search was conducted using the following strings in the title/abstract/Keyword: “sorghum AND polyphenols, sorghum AND phenolic acids, phenolic acids AND antioxidant activity, phenolic acids AND therapeutic, ferulic acid AND therapeutic, ferulic acid AND antioxidant, Ferulic acid AND anticancer, ferulic acid AND antidiabetic, ferulic acid AND cardiovascular diseases, ferulic acid AND gastrointestinal diseases, p-coumaric acid AND therapeutic, p-coumaric acid AND antioxidant, p-coumaric acid AND anticancer, p-coumaric acid AND antidiabetic, p-coumaric acid AND cardiovascular diseases, p-coumaric acid AND gastrointestinal diseases, protocatechuic acid AND therapeutic, protocatechuic acid AND antioxidant, protocatechuic acid AND anticancer, protocatechuic acid AND antidiabetic, protocatechuic acid AND cardiovascular diseases, protocatechuic acid AND gastrointestinal diseases.” Results were obtained from the year 1983 to 2017. Depending on the title and the abstract, the most relevant articles were analyzed and references in the obtained publications were analyzed too, to identify other relevant publications.
| Phenolic Compounds in Sorghum Grain|| |
The bioactive effects of sorghum grain are mainly related to its antioxidant, anticarcinogenic, hypolipidemic, antimutagenic, antimicrobial, and antitumor activities.,,,,,,, A very large number of studies associate these beneficial effects of sorghum with molecules belonging to the group of polyphenols. In fact, sorghum contains high levels of phenolic acids, flavonoids, and anthocyanins, which represent the main groups of polyphenols present in this cereal., In sorghum grains, polyphenols are mainly located in the layers of pericarp, testa, and aleurone.
| Phenolic Acids in Sorghum Grain|| |
Phenolic acids are phenylpropanoids characterized by an aromatic ring attached to three carbon side chains. The phenolic acids are mostly derived from cinnamic acid and benzoic acid. They are generally subdivided into hydroxybenzoic and hydroxycinnamic acids. Despite their distribution in different organ of sorghum plants, the phenolic acids are mainly present in the stalks, sheaths, and grains. In grains, several phenolic acids have been reported and are listed in [Table 2] and [Figure 1]. The predominant phenolic acids reported are ferulic acid, p-coumaric acid, and protocatechuic acid.
Ferulic acid (FA, 4-hydroxy-3-methoxycinnamic acid) is widely distributed in plants and was first isolated from Ferula foetida in 1866. FA which is one of the most abundant phenolic acids in sorghum grains, is reported to possess different biological effects.
p-Coumaric acid (p-CA, 4-Hydroxycinnamic acid) is one of the three isomers of coumaric acid (o-coumaric, m-coumaric, and p-coumaric acids). p-CA is the most abundant isomer in nature and it is synthesized from cinnamic acid by the action of 4-cinnamic acid hydroxylase. This molecule is widely found in fruits, vegetables, and cereals such as sorghum, maize, wheat, and oats. It has been shown that p-CA provides protection against different pathological conditions.
Protocatechuic acid (PCA, 3,4-dihydroxybenzoic acid) is a major phenolic acid that is found in fruits, medicinal plants, nuts, and vegetables. PCA has been shown to have different beneficial health activities which are almost related to its antioxidant, anticancer, and antidiabetic activities.,,
In this review, we will focus solely on these three molecules because of their beneficial pharmacological properties.
| Main Pharmacological Mechanisms of Most Relevant Phenolic Acids in Sorghum|| |
Different studies have outlined the importance of the phenolic acids in the prevention of different chronic diseases, including cancers, diabetes, cardiovascular diseases, and gastrointestinal diseases. In this section, we discuss the most relevant pharmacological effects of the main phenolic acid compounds found in sorghum grains and which are ferulic acid, p-coumaric acid, and protocatechuic acid.
Many studies reported the high antioxidant capacities and the excellent free radical scavenger effects of the FA, p-CA, and PCA.,,,,, Their antioxidant effect may be directly related to the number and the position of the hydroxyl groups.,,,,
FA's ability to reduce oxidative stress has been shown to have a therapeutic effect on several chronic diseases associated with oxidative damage. In fact, it was reported that FA might play a neuroprotective role. This effect has been observed in a study carried out on rats, in which, FA significantly reduces cerebral infarction and neurological deficit through the inhibition of superoxide radicals. It was also reported that FA has a protective and therapeutic effect on diabetic nephropathy by reducing oxidative stress and inflammation. Recently, it was suggested that the beneficial effects of FA might also be due to the aptitude of this phenolic acid to interact with several cellular mechanisms (including regulation of signaling pathways), which can be simultaneously and synergistically implicated in its biologic effect.
As for FA, the antioxidant effect of p-CA makes from this compound an important protective molecule against different diseases. In fact, p-CA has shown a protective effect against heart disease through its ability to enhance the resistance of low-density lipoproteins to cholesterol oxidation and to reduce lipid peroxidation., Another study conducted by Masek et al. has confirmed the antioxidant activity of p-CA and also showed its ability to reduce iron and copper ions. Compared to ferulic acid, p-CA showed similar or even higher ability to eliminate reactive oxygen species in human lung (A549) and colon adenocarcinoma (HT29-D4) cell lines.
It is currently well established that PCA is a natural compound with high antioxidant capacity. The activity of PCA is due to its capacity of sequestration of transition metals ions that are responsible for free radicals and its potent radical scavenging ability. The antioxidant activity of PCA is associated with its potential to prevent oxidative damage of the DNA and to decrease lipid peroxidation in in vitro studies., In the other hand, some studies showed that PCA exerts an indirect antioxidant effects through the induction of genes that are involved in the endogenous defense system. This includes antioxidant enzymes such as catalase, superoxide dismutase, glutathione (GSH) reductase, and GSH peroxidase as well as nonenzymatic antioxidants which play an important role in protecting the cell against oxidative damage.,
Free radicals play an important role in cancer, especially in invasive and metastatic tumors., Thereby, phenolic acids, such as FA, p-CA, and PCA have been studied for a long time for their probable beneficial effects on many diseases including cancer, due to their radical scavenging properties.
Various studies have shown the anticancer effect of FA against different types of cancer such as colon, lung, osteosarcoma, and melanoma cancer.,,, The study conducted by Zhang et al. showed that FA leads to decreased viability, increased apoptosis, and suppression of metastatic potential in the MDA-MB-231 breast cancer cell line. showed that FA could also significantly decrease the cell viability of osteosarcoma through the apoptosis pathway. In fact, in this study, the authors showed that FA activates the pro-apoptotic genes, caspase-3, and Bax and inactivates the anti-apoptotic gene, Bcl-2. The anticancer activity of FA was also attributed to its ability to inhibit cyclooxygenase-2, which is overexpressed in several types of cancer and which is considered as a target for the development of the anticancer drug. It was also reported that FA might inhibit proliferation and induce apoptosis via inhibition of the proliferation-related pathway, phosphoinositide 3-kinase/protein kinase B (Akt), in osteosarcoma cells or through the release of cytochrome C.,
The in vitro evaluation of the activity of p-CA on the growth of certain cell lines showed a moderate inhibition capacity of this molecule. However, this phenolic acid leads to a significant decrease in the viability of neuroblastoma N2a cells and cancer stem cells ,, highlighted the effect of p-CA on the colorectal cancer cells and showed an effective activity of this compound in killing cancer cells. In another study, Kong et al. showed that the effect of p-CA is due to the inhibition of the signaling pathways responsible for angiogenesis (Akt and extracellular-regulated kinase), and also, to the reduction of the expression of two of the most important angiogenic factors that stimulate proliferation, migration, and tube formation of endothelial cells (vascular endothelial growth factor-A and basic fibroblast growth factor).
Many published studies highlighted the antiproliferative capacity of PCA on multiple human cell lines such as gastric adenocarcinoma cells MKN45, breast cancer cells T47D and lung cancer cells A549 and H3255,,, reported that PCA has a protective effect and can prevent osteoclast differentiation via regulating inflammation and oxidative stress and by inducing apoptosis in RAW264.7 murine macrophage cells. PCA was also evaluated for its role as a chemopreventive agent in different types of induced carcinogenesis in laboratory mice and rats., Moreover, PCA can also suppress the expression of the necrosis factor (tumor necrosis factor alpha which is involved in carcinogenesis. Moreover, PCA may affect enzyme activities implicated in carcinogen metabolism and also counteracts the effect of reactive intermediate metabolites by preventing their binding to DNA and thus, preventing DNA mutations which may lead to tumor initiation. In another study, PCA showed to inhibit the progression of cancer cells through the repression of migration of B16/F10 melanoma cells to the liver in mice.
FA, p-CA, and PCA, which are widely present in fruits, vegetables and cereals are good competitors for the actual drug treatments used against diabetes and its complications.,,,
Concerning the effects of FA, a study conducted by Balasubashini et al. showed that treatment of diabetic rats with this phenolic acid decreased blood glucose levels and free fatty acids, and increased reduced GSH levels in the liver of these diabetic animals. In another study, Ohnishi et al. demonstrated that FA also inhibited lipid peroxidation in the brown adipose tissue of diabetic mice. Other studies have shown that FA could regenerate pancreatic β-cells and regulate glucose levels by increasing the activity of glucokinase and the production of glycogen ,, showed that FA has protective and therapeutic effects on diabetic nephropathy through reducing inflammation and oxidative stress. Recently, Sompong et al. showed that FA inhibited methylglyoxal-induced protein glycation and oxidative protein denaturation in bovine serum albumin. These authors also showed that FA reduced mg-induced cell apoptosis in pancreatic β-cells.
It was observed that p-CA might regulate the expression of adiponectin (participate in the modulation of insulin sensitivity) in 3T3-L1 adipocytes after exposure for 24 h to this molecule. It was also reported that p-CA and its conjugates might bind to glucosidases and thus decrease their enzymatic activities. In diabetic rats, p-CA improves plasma insulin levels and also regulates glucose levels. In 2016, have shown that the anti-diabetic activity of p-CA plays a protective role in the β-pancreatic of diabetic rat cells by improving the antioxidant status and reducing ROS-induced oxidative stress. These authors suggest that p-CA regulates the glucose metabolism through activation of the glucose transporter (GLUT-2) in the pancreas. The study conducted by  on diabetic rats to evaluate the effect of p-CA on type 2 diabetes-induced neurodegeneration, revealed that the treatment of these animals with p-CA significantly improved glucose tolerance and decreased the brain oxidative stress of these rats. The p-CA was also responsible for the decrease of inflammation and the inhibition of apoptosis in the hippocampus, suggesting a beneficial role of this molecule in the attenuation of type 2 diabetes-induced neurodegeneration.
It was highlighted that PCA is a phenolic compound which is widely present in plant foods and medicinal plants and which may be useful for diabetic patients., The treatment of diabetic rats by PCA for 45 days revealed that this molecule could prevent the increase in blood glucose by increasing the secretion of insulin and the glycogen synthesis enzymes. The same study demonstrated that PCA could alleviate hyperlipidemia. PCA has also the ability to stimulate the insulin signaling pathway by increasing the GLUT-4 translocation and glucose uptake in human adipocytes. The study conducted by Semaming et al. showed that PCA is an important molecule for diabetic patients because of its ability to reduce vascular complications, which is mainly due to its antioxidant activity. In fact, in a study conducted in vivo, Lin et al. showed that PCA dietary supplement leads to a decrease in the level of hepatic and cardiac triglycerides. PCA also decreases the oxidative and inflammatory stress in the kidneys and heart of the diabetic mice used for this study.
It was reported that salt of FA can: (1) inhibit myocardial cell death after anoxia/reoxygenation by reducing Ca 2+ overload, (2) reduce the area of experimental myocardial infarction, and (3) decrease the oxygen consumption of guinea pig myocardial homogenates. Salt of FA also has a clear protective effect in experimental myocardial ischemia. In fact, the mechanisms of salvia fruticosa-induced protection from myocardial ischemia/reperfusion injury appear to involve inhibition of arachidonic acid metabolism, inhibition of the oxygen free radicals and of subsequent lipid peroxidation. Another study conducted by Carpita et al. has confirmed that the blood pressure was decreased in both stroke-prone spontaneously hypertensive rats (SHRSP) and spontaneously hypertensive rats (SHR) with a maximum effect (−34 mmHg) after 2 h of oral intake of FA (1–100 mg/kg body weight).
p-coumaric acid (p-CA) with its potent antioxidant potential shows potential cardioprotective effects against Doxorubicin (DOX)-induced oxidative stress in rat's heart  and attenuates ROS-induced cardiomyoblast damage when pre-treated or co-treated with DOX. In another study, the combination of PC and naringenin may act as a hydrogen-donating radicals scavenger by scavenging lipid alkoxyl and peroxyl radical and protect myocardium from DOX-induced injury. It was reported that pCA exerts a protective effect on the alterations in the gene-expression profile in sodium arsenite-induced cardiotoxicity in rats. p CA also increased the myocardial expression of Bax, caspase-8, caspase-9, and Fas genes and showed a decrease in the myocardial expression of Bcl-2 and Bcl-xL genes.
PCA has been reported to improve cardiac function, cardiac autonomic balance and prevent cardiac mitochondrial dysfunction in STZ-induced type 1 diabetes mellitus rats. PCA has also shown beneficial effects in acute myocardial infarction with propranolol in dogs. Previous studies indicate that PCA could reduce myocardial infarcts and interfere with the following MI/R pathogenic procedures including the inflammatory response, platelet aggregation, and cardiomyocyte apoptosis. 2, 3, 7, 8-Tetrachlorodibenzo-p-dioxin cardiotoxicity in 3–4 months old rats was studied and protocatechuic acid treatment at the dose of 100 mg/kg for 45 days was found to decrease the levels of Thiobarbituric acid reactive substances, while increasing those of GSH, catalase, GSH peroxidase, and superoxide dismutase.
It was observed that FA pretreatment significantly attenuated the effects of heat stress on the small intestine, including the increased FD4 permeability, disrupted tight junctions and microvilli structure and reduced occludin, ZO-1, and E-cadherin expression , suggested that the gastrokinetic activity of FA may be partially mediated via interference with nitric oxide (NO) production, and NO plays a key role in the regulation of gastrointestinal motility by its smooth muscle relaxing and vasodilating activity. In another study  have shown that effects caused by cisplatin (plays an important role in the treatment of malignant diseases), namely, severe nausea, and vomiting, accompanying gastrointestinal symptoms such as abdominal discomfort in the patients were significantly reversed by pretreatment by ferulic acid. The beneficial effect of FA could be attributed at least partly to its stimulant effect on the gastrointestinal tract and its antioxidant effect.
p-CA has been reported to decreases effectively oxidative DNA damage in rat colonic mucosa. p-CA exerts this effect by the increased expression of Glutathione S-Transferase Mu 2 (GST-M2), an important isoform of GST , have observed that p-CA inhibits the lesion area of ethanol-induced ulcer, indomethacin-induced gastric ulcers, stress-induced gastric ulcers by 83.3%, 55%, and 73%, respectively.,,, demonstrated that the high concentration of p-CAconjugates reaching the colon produces various physiological actions aimed at the colon microbiota. The colon microbiota plays a role in the trophic effects on intestinal epithelia, immunological function, and protection against invasion of alien microbes and colon cancer  have observed that treatment using doses of 50 and 250 mg/kg p-coumaric significantly diminished the lesion index, the total area of the lesion and the percentage of the lesion in comparison with the negative control groups (omeprazole or cimetidine).
Kore et al. have demonstrated that PCA ethyl ester administered at the dose of (30 mg/kg and 60 mg/kg i. p.) 30 min before ulcer induction was found to possess the significant antiulcer property, and the ulcer index was significantly less in comparison control animals. The mechanism of action of PCA ethyl ester may be due to strengthening the gastric mucosa thereby enhancing mucosal defense. In another study, Ma et al. have observed that PCA pretreatment had significant restraining effects on p66shc messenger RNA expression and protein phosphorylation after intestinal I/R in the intestine accompanied by p66shc-related oxidative stress regulators and apoptotic protein alteration. It was also reported that severe intestinal mucosal lesions occurred after intestinal I/R decreased significantly by PCA pretreatment, suggesting that PCA can improve morphological alterations in the intestinal mucosa in a murine model of intestinal I/R.
| Other Beneficial Effects of Ferulic Acid P-Coumaric Acid, and Protocatechuic Acid|| |
The list of the beneficial effects of FA, p-CA, and PCA that was given above is not exhaustive. In fact, these natural molecules have a broad spectrum of activities against several other diseases and have many other beneficial activities.
FA, p-CA, and PCA have been reported to exhibit a wide range of antimicrobial activities against bacteria and yeasts.,,,,,,,, One of the possible mechanisms leading to this effect is through causing changes in cellular morphology and cell membrane dysfunction., The antibacterial activity may also be due to the capability of these compounds to stop bacterial growth and their ability to enhance the synergistic effects of antibiotics which reduce the possibility of antibiotic resistance as it was reported for the PCA.
Another important effect of these three molecules concerns their anti-inflammatory activity which makes from them potent molecules for the regulation of the inflammatory response.,,, Moreover, studies reported on the effect of FA and p-CA on UV radiation have shown that these molecules are potent UV absorber.,, In addition, it has been shown that FA pretreatment of human dermal fibroblasts protects cells against UV-A irradiation by inducing proliferation and progression of cell cycle.
FA, p-CA, and PCA have also been proved to have anti-atherogenic and anti-atherosclerotic effects.,,, The FA and p-CA were also reported to have the ability to inhibit platelet aggregation via the reduction of thromboxane B2 production and the inhibition of platelet–leucocyte interactions.,,,
| Concluding Remarks|| |
Dietary polyphenols as one of the main components of sorghum bicolor grains have demonstrated multiple beneficial activities against several human diseases. In fact, analysis of the published work indicated that the efficacy of three phenolic acids reported in sorghum grains which are FA, p-CA, and PCA in the treatment of gastrointestinal disorders, cardiovascular, diabetic, and cancer diseases and as potent molecules to be used as antioxidant, antimicrobial and anti-inflammatory. Therefore, a higher intake of Sorghum bicolor can ensure a healthy diet and would have good beneficial pharmacological properties throughout its grain. Thus, the addition of sorghum to human diet as other cereals is of great importance. Further studies in future will be important to evaluate other compound extracts from grain, leaf as well as the root of sorghum plants.
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| References|| |
Rao SS, Patil JV, Prasad PV, Reddy DC, Mishra JS, Umakanth AV, et al
. Sweet sorghum planting effects on stalk yield and sugar quality in semi-arid tropical environment. Agron J 2013;105:1458-65.
Food and Agriculture Organization of the United States. Food Outlook Biannual Report on global Food Markets. Roma: Industry Training Authority; 2015. p. 58-63.
Khoddami A, Mohammadrezaei M, Roberts TH. Effects of sorghum malting on colour, major classes of phenolics and individual anthocyanins. Molecules 2017;22. pii: E1713.
Dicko MH, Gruppen H, Traoré AS, Voragen AG, van Berkel WJ. Sorghum grain as human food in Africa: Relevance of content of starch and amylase activities. Afr J Biotechnol 2006;5:384-95.
Jacob AA, Fidelis AE, Salaudeen KO, Queen KR. Sorghum: Most under-utilized grain of the Semi-Arid Africa. Sch J Agr Sci 2013;3:147-53.
Singh PK, Kumar S, Bhat ZF, Kumar P. Effect of Sorghum bicolor and clove oil on the quality characteristics and storage quality of aerobically packaged chevon cutlets. Nutr Food Sci 2015;45:145-63.
Awada F. Assesment of Sorghum Response to Nitrogen Availability. Paris-Saclay: Doctoral dissertation; 2016.
Bartwal A, Mall R, Lohani P, Guru SK, Arora S. Role of secondary metabolites and brassinosteroids in plant defense against environmental stresses. J Plant Growth Regul 2012;32:216-32.
Kaur R, Uppal SK. Structural characterization and antioxidant activity of lignin from sugarcane bagasse. Colloid Polym Sci 2015;293:2585-92.
Verpoorte R, Contin A, Memelink J. Biotechnology for the production of plant secondary metabolites. Phytochem Rev 2002;1:13-25.
Agrawal AA, Weber MG. On the study of plant defence and herbivory using comparative approaches: How important are secondary plant compounds. Ecol Lett 2015;18:985-91.
Michalak A. Phenolic compounds and their antioxidant activity in plants growing under heavy metal stress. Pol J Environ Stud 2006;4:523-30.
Taiz L, Zeiger E. Plant Physiology. 5th
ed. Massachusetts: Sinauer Associates Inc; 2010.
Olivoto T, Nardino M, Carvalho IR, Follmann DN, Szareski VIJ, Ferrari M, et al
. Plant secondary metabolites and its dynamical systems of induction in response to environmental factors: A review. Afr J Agr Res 2017;12:71-84.
Kittakoop P, Mahidol C, Ruchirawat S. Alkaloids as important scaffolds in therapeutic drugs for the treatments of cancer, tuberculosis, and smoking cessation. Curr Top Med Chem 2014;14:239-52.
Takemura M, Tanaka R, Misawa N. Pathway engineering for the production of β-amyrin and cycloartenol in escherichia coli-a method to biosynthesize plant-derived triterpene skeletons in E. Coli
. Appl Microbiol Biotechnol 2017;101:6615-25.
Lamarti A, Badoc A, Deffieux G, Carde JP. Biogenesis of monoterpenes: The isoprenic chain. Bull Soc Pharm Bordeaux 1994;133:79-99.
Bakkali F, Averbeck S, Averbeck D, Idaomar M. Biological effects of essential oils – A review. Food Chem Toxicol 2008;46:446-75.
Soriano IR, Riley IT, Potter MJ, Bowers WS. Phytoecdysteroids: A novel defense against plant-parasitic nematodes. J Chem Ecol 2004;30:1885-99.
Veitch GE, Boyer A, Ley SV. The azadirachtin story. Angew Chem Int Ed Engl 2008;47:9402-29.
Gómez-Caravaca AM, Gómez-Romero M, Arráez-Román D, Segura-Carretero A, Fernández-Gutiérrez A. Advances in the analysis of phenolic compounds in products derived from bees. J Pharm Biomed Anal 2006;41:1220-34.
Ali MB, Hahn EJ, Paek KY. Methyl jasmonate and salicylic acid induced oxidative stress and accumulation of phenolics in panax ginseng bioreactor root suspension cultures. Molecules 2007;12:607-21.
Thakur A, Singla R, Jaitak V. Coumarins as anticancer agents: A review on synthetic strategies, mechanism of action and SAR studies. Eur J Med Chem 2015;101:476-95.
Homoki JR, Nemes A, Fazekas E, Gyémánt G, Balogh P, Gál F, et al.
Anthocyanin composition, antioxidant efficiency, and α-amylase inhibitor activity of different hungarian sour cherry varieties (Prunus cerasus
L.). Food Chem 2016;194:222-9.
Salawu SO, Alao OF, Faloye OF, Akindahunsi AA, Boligon AA, Athayde ML. Antioxidant potential of phenolic-rich two varieties of Nigerian local rice and their anti-cholinesterase activities after in vitro
digestion. Nutr Food Sci 2016;46:171-89.
Boveris AD, Galatro A, Sambrotta L, Ricco R, Gurni AA, Puntarulo S, et al.
Antioxidant capacity of a 3-deoxyanthocyanidin from soybean. Phytochemistry 2001;58:1097-105.
Kwak CS, Park SC, Lim SJ, Kim SA, Lee MS. Antioxidative and antimutagenic effects of Korean buckwheat, sorghum, millet and job's tears. J Korean Soc Food Sci Nutr 2004;33:921-9.
Dicko MH, Gruppen H, Traore AS, van Berkel WJ, Voragen AG. Evaluation of the effect of germination on phenolic compounds and antioxidant activities in sorghum varieties. J Agric Food Chem 2005;53:2581-8.
Choi Y, Jeong HS, Lee J. Antioxidant activity of methanolic extracts from some grains consumed in Korea. Food Chem 2007;103:130-8.
Kil HY, Seong ES, Ghimire BK, Chung IM, Kwon SS, Goh EJ, et al
. Antioxidant and antimicrobial activities of crude sorghum extract. Food Chem 2009;115:1234-9.
Chung IM, Yeo MA, Kim SJ, Kim MJ, Park DS, Moon HI, et al.
Antilipidemic activity of organic solvent extract from sorghum bicolor on rats with diet-induced obesity. Hum Exp Toxicol 2011;30:1865-8.
Shih CH, Siu SO, Ng R, Wong E, Chiu LC, Chu IK, et al.
Quantitative analysis of anticancer 3-deoxyanthocyanidins in infected sorghum seedlings. J Agric Food Chem 2007;55:254-9.
Zbasnik R, Carr T, Weller C, Hwang KT, Wang L, Cuppett S, et al.
Antiproliferation properties of grain sorghum dry distiller's grain lipids in caco-2 cells. J Agric Food Chem 2009;57:10435-41.
Dykes L, Rooney LW, Waniska RD, Rooney WL. Phenolic compounds and antioxidant activity of sorghum grains of varying genotypes. J Agric Food Chem 2005;53:6813-8.
Dykes L, Seitz LM, Rooney WL, Rooney LW. Flavonoid composition of red sorghum genotypes. Food Chem 2009;116:313-7.
Awika JM, McDonough CM, Rooney LW. Decorticating sorghum to concentrate healthy phytochemicals. J Agric Food Chem 2005;53:6230-4.
Taylor JR, Belton PS, Beta T, Duodu KG. Increasing the utilisation of sorghum, millets and pseudocereals: Developments in the science of their phenolic phytochemicals, biofortification and protein functionality. J Cereal Sci 2014;59:257-75.
Alban T, Manuel V. Phenolic Compounds of Sorghum, their Chemopreventive Properties and Absorption (Doctoral dissertation); 2013.
Hahn DH, Roonet LW, Faubion JM. Sorghum phenolic acids, their HPLC separation and their relation to fungal resistance. Cereal Chem 1983;60:255-9.
Subba Rao MV, Muralikrishna G. Evaluation of the antioxidant properties of free and bound phenolic acids from native and malted finger millet (ragi, Eleusine coracana
indaf-15). J Agric Food Chem 2002;50:889-92.
Awadelkareem AM, Muralikrishna G, El Tinay AH, Mustafa AI. Characterization of tannin and study of in vitro
protein digestibility and mineral profile of Sudanese and Indian sorghum cultivars. Pak J Nutr 2009;8:469-76.
McDonough CM, Rooney LW, Earp CF. Structural characteristics of Eleusine corocana
(finger millet) using scanning electron and fluorescence microscopy. Food Struct 1986;5:9.
Waniska RD, Poe JH, Bandyopadhyay R. Effects of growth conditions on grain molding and phenols in sorghum caryopsis. J Cereal Sci 1989;10:217-55.
Dykes L, Rooney LW. Sorghum and millet phenols and antioxidants. J Cereal Sci 2006;44:236-51.
Sgarbossa A, Giacomazza D, di Carlo M. Ferulic acid: A Hope for Alzheimer's disease therapy from plants. Nutrients 2015;7:5764-82.
Kong CS, Jeong CH, Choi JS, Kim KJ, Jeong JW. Antiangiogenic effects of p-coumaric acid in human endothelial cells. Phytother Res 2013;27:317-23.
Lin WL, Hsieh YJ, Chou FP, Wang CJ, Cheng MT, Tseng TH, et al.
Hibiscus protocatechuic acid inhibits lipopolysaccharide-induced rat hepatic damage. Arch Toxicol 2003;77:42-7.
Min SW, Ryu SN, Kim DH. Anti-inflammatory effects of black rice, cyanidin-3-O-beta-D-glycoside, and its metabolites, cyanidin and protocatechuic acid. Int Immunopharmacol 2010;10:959-66.
Varì R, D'Archivio M, Filesi C, Carotenuto S, Scazzocchio B, Santangelo C, et al.
Protocatechuic acid induces antioxidant/detoxifying enzyme expression through JNK-mediated nrf2 activation in murine macrophages. J Nutr Biochem 2011;22:409-17.
Kakkar S, Bais S. A review on protocatechuic acid and its pharmacological potential. ISRN Pharmacol 2014;2014:952943.
Nagai N, Kotani S, Mano Y, Ueno A, Ito Y, Kitaba T, et al
. Ferulic qcid suppresses Amyloid β production in the human lens epithelial cell stimulated with hydrogen peroxide. Biomed Res Int 2017;5343010.
Gani A, Wani SM, Masoodi FA, Hameed G. Whole-grain cereal bioactive compounds and their health benefits: A review. J Food Process Technol 2012;3:146-56.
Roy AJ, Stanely Mainzen Prince P. Preventive effects of p-coumaric acid on cardiac hypertrophy and alterations in electrocardiogram, lipids, and lipoproteins in experimentally induced myocardial infarcted rats. Food Chem Toxicol 2013;60:348-54.
Stojković D, Petrović J, Soković M, Glamočlija J, Kukić-Marković J, Petrović S, et al
. In situ
antioxidant and antimicrobial activities of naturally occurring caffeic acid, p-coumaric acid and rutin, using food systems. J Sci Food Agric 2013;93:3205-8.
Masek A, Chrzescijanska E, Latos M. Determination of antioxidant activity of caffeic acid and p-coumaric acid by using electrochemical and spectrophotometric assays. Int J Electrochem Sci 2016;11:10644-58.
Balasundram N, Sundram K, Samman S. Phenolic compounds in plants and agri-industrial by-products: Antioxidant activity, occurrence, and potential uses. Food Chem 2006;99:191-203.
Kadoma Y, Fujisawa S. A comparative study of the radical-scavenging activity of the phenolcarboxylic acids caffeic acid, p-coumaric acid, chlorogenic acid and ferulic acid, with or without 2-mercaptoethanol, a thiol, using the induction period method. Molecules 2008;13:2488-99.
Aguilera Y, Dueñas M, Estrella I, Hernández T, Benitez V, Esteban RM, et al.
Phenolic profile and antioxidant capacity of chickpeas (Cicer arietinum
L.) as affected by a dehydration process. Plant Foods Hum Nutr 2011;66:187-95.
Cheng CY, Ho TY, Lee EJ, Su SY, Tang NY, Hsieh CL, et al.
Ferulic acid reduces cerebral infarct through its antioxidative and anti-inflammatory effects following transient focal cerebral ischemia in rats. Am J Chin Med 2008;36:1105-19.
Choi R, Kim BH, Naowaboot J, Lee MY, Hyun MR, Cho EJ, et al.
Effects of ferulic acid on diabetic nephropathy in a rat model of type 2 diabetes. Exp Mol Med 2011;43:676-83.
de Oliveira Silva E, Batista R. Ferulic acid and naturally occurring compounds bearing a feruloyl moiety: A Review on their structures, occurrence, and potential health benefits. Compr Rev Food Sci Food Saf 2017;16:580-616.
Garrait G, Jarrige JF, Blanquet S, Beyssac E, Cardot JM, Alric M, et al.
Gastrointestinal absorption and urinary excretion of trans-cinnamic and p-coumaric acids in rats. J Agric Food Chem 2006;54:2944-50.
Nasr Bouzaiene N, Kilani Jaziri S, Kovacic H, Chekir-Ghedira L, Ghedira K, Luis J, et al.
The effects of caffeic, coumaric and ferulic acids on proliferation, superoxide production, adhesion and migration of human tumor cells in vitro
. Eur J Pharmacol 2015;766:99-105.
Li X, Wang X, Chen D, Chen S. Antioxidant activity and mechanism of protocatechuic acid in vitro
. Funct Food Health Dis 2011;7:232-44.
Valentova K, Cvak L, Muck A, Ulrichova J, Simanek V. Antioxidant activity of extracts from the leaves of Smallanthus sonchifolius
. Eur J Nutr 2003;42:61-6.
Schmeda-Hirschmann G, Tapia A, Theoduloz C, Rodríguez J, López S, Feresin GE, et al.
Free radical scavengers and antioxidants from tagetes mendocina. Z Naturforsch C 2004;59:345-53.
Adefegha SA, Oboh G, Omojokun OS, Adefegha OM. Alterations of Na +
-ATPase, cholinergic and antioxidant enzymes activity by protocatechuic acid in cadmium-induced neurotoxicity and oxidative stress in Wistar rats. Biomed Pharmacother 2016;83:559-68.
Loo G. Redox-sensitive mechanisms of phytochemical-mediated inhibition of cancer cell proliferation (review). J Nutr Biochem 2003;14:64-73.
Barone E, Calabrese V, Mancuso C. Ferulic acid and its therapeutic potential as a hormetin for age-related diseases. Biogerontology 2009;10:97-108.
Jayaprakasam B, Vanisree M, Zhang Y, Dewitt DL, Nair MG. Impact of alkyl esters of caffeic and ferulic acids on tumor cell proliferation, cyclooxygenase enzyme, and lipid peroxidation. J Agric Food Chem 2006;54:5375-81.
Janicke B, Hegardt C, Krogh M, Onning G, Akesson B, Cirenajwis HM, et al.
The antiproliferative effect of dietary fiber phenolic compounds ferulic acid and p-coumaric acid on the cell cycle of caco-2 cells. Nutr Cancer 2011;63:611-22.
Yang GW, Jiang JS, Lu WQ. Ferulic acid exerts anti-angiogenic and anti-tumor activity by targeting fibroblast growth factor receptor 1-mediated angiogenesis. Int J Mol Sci 2015;16:24011-31.
Wang T, Gong X, Jiang R, Li H, Du W, Kuang G, et al.
Ferulic acid inhibits proliferation and promotes apoptosis via blockage of PI3K/Akt pathway in osteosarcoma cell. Am J Transl Res 2016;8:968-80.
Zhang X, Lin D, Jiang R, Li H, Wan J, Li H, et al.
Ferulic acid exerts antitumor activity and inhibits metastasis in breast cancer cells by regulating epithelial to mesenchymal transition. Oncol Rep 2016;36:271-8.
Zhang XD, Wu Q, Yang SH. Ferulic acid promoting apoptosis in human osteosarcoma cell lines. Pak J Med Sci 2017;33:127-31.
Cione E, Tucci P, Senatore V, Perri M, Trombino S, Iemma F, et al.
Synthesized esters of ferulic acid induce release of cytochrome c from rat testes mitochondria. J Bioenerg Biomembr 2008;40:19-26.
Shailasree S, Venkataramana M, Niranjana SR, Prakash HS. Cytotoxic effect of p-coumaric acid on neuroblastoma, N2a cell via generation of reactive oxygen species leading to dysfunction of mitochondria inducing apoptosis and autophagy. Mol Neurobiol 2015;51:119-30.
Min SJ, Lim JY, Kim HR, Kim SJ, Kim Y. Sasa quelpaertensis leaf extract inhibits colon cancer by regulating cancer cell stemness in vitro
and in vivo
. Int J Mol Sci 2015;16:9976-97.
Roy N, Narayanankutty A, Nazeem PA, Valsalan R, Babu TD, Mathew D, et al.
Plant phenolics ferulic acid and p-coumaric acid inhibit colorectal cancer cell proliferation through EGFR down-regulation. Asian Pac J Cancer Prev 2016;17:4019-23.
Hudson EA, Dinh PA, Kokubun T, Simmonds MS, Gescher A. Characterization of potentially chemopreventive phenols in extracts of brown rice that inhibit the growth of human breast and colon cancer cells. Cancer Epidemiol Biomarkers Prev 2000;9:1163-70.
Kampa M, Alexaki VI, Notas G, Nifli AP, Nistikaki A, Hatzoglou A, et al.
Antiproliferative and apoptotic effects of selective phenolic acids on T47D human breast cancer cells: Potential mechanisms of action. Breast Cancer Res 2004;6:R63-74.
Tsao SM, Hsia TC, Yin MC. Protocatechuic acid inhibits lung cancer cells by modulating FAK, MAPK, and NF-κB pathways. Nutr Cancer 2014;66:1331-41.
Wu YX, Wu TY, Xu BB, Xu XY, Chen HG, Li XY, et al.
Protocatechuic acid inhibits osteoclast differentiation and stimulates apoptosis in mature osteoclasts. Biomed Pharmacother 2016;82:399-405.
Nakamura Y, Torikai K, Ohto Y, Murakami A, Tanaka T, Ohigashi H, et al.
A simple phenolic antioxidant protocatechuic acid enhances tumor promotion and oxidative stress in female ICR mouse skin: Dose-and timing-dependent enhancement and involvement of bioactivation by tyrosinase. Carcinogenesis 2000;21:1899-907.
Peiffer DS, Zimmerman NP, Wang LS, Ransom BW, Carmella SG, Kuo CT, et al.
Chemoprevention of esophageal cancer with black raspberries, their component anthocyanins, and a major anthocyanin metabolite, protocatechuic acid. Cancer Prev Res (Phila) 2014;7:574-84.
Zhou-Stache J, Buettner R, Artmann G, Mittermayer C, Bosserhoff AK. Inhibition of TNF-alpha induced cell death in human umbilical vein endothelial cells and jurkat cells by protocatechuic acid. Med Biol Eng Comput 2002;40:698-703.
Ignatowicz E, Balana B, Vulimiri SV, Szaefer H, Baer-Dubowska W. The effect of plant phenolics on the formation of 7,12-dimethylbenz[a] anthracene-DNA adducts and TPA-stimulated polymorphonuclear neutrophils chemiluminescence in vitro
. Toxicology 2003;189:199-209.
Lin HH, Chen JH, Chou FP, Wang CJ. Protocatechuic acid inhibits cancer cell metastasis involving the down-regulation of Ras/Akt/NF-κB pathway and MMP-2 production by targeting RhoB activation. Br J Pharmacol 2011;162:237-54.
Kay CD, Kroon PA, Cassidy A. The bioactivity of dietary anthocyanins is likely to be mediated by their degradation products. Mol Nutr Food Res 2009;53 Suppl 1:S92-101.
Scazzocchio B, Varì R, Filesi C, Del Gaudio I, D'Archivio M, Santangelo C, et al.
Protocatechuic acid activates key components of insulin signaling pathway mimicking insulin activity. Mol Nutr Food Res 2015;59:1472-81.
Balasubashini MS, Rukkumani R, Viswanathan P, Menon VP. Ferulic acid alleviates lipid peroxidation in diabetic rats. Phytother Res 2004;18:310-4.
Ohnishi M, Matuo T, Tsuno T, Hosoda A, Nomura E, Taniguchi H, et al.
Antioxidant activity and hypoglycemic effect of ferulic acid in STZ-induced diabetic mice and KK-Ay mice. Biofactors 2004;21:315-9.
Jung EH, Kim SR, Hwang IK, Ha TY. Hypoglycemic effects of a phenolic acid fraction of rice bran and ferulic acid in C57BL/KsJ-db/db mice. J Agric Food Chem 2007;55:9800-4.
Mandal S, Barik B, Mallick C, De D, Ghosh D. Therapeutic effect of ferulic acid, an ethereal fraction of ethanolic extract of seed of Syzygium cumini
against streptozotocin-induced diabetes in male rat. Methods Find Exp Clin Pharmacol 2008;30:121-8.
Choi S, Il Kim H, Hag Park S, Jung Lee M, Yeoul Jun J, Lee Kim H, et al.
Endothelium-dependent vasodilation by ferulic acid in aorta from chronic renal hypertensive rats. Kidney Res Clin Pract 2012;31:227-33.
Sompong W, Cheng H, Adisakwattana S. Ferulic acid prevents methylglyoxal-induced protein glycation, DNA damage, and apoptosis in pancreatic β-cells. J Physiol Biochem 2017;73:121-31.
Hsu CL, Yen GC. Effects of flavonoids and phenolic acids on the inhibition of adipogenesis in 3T3-L1 adipocytes. J Agric Food Chem 2007;55:8404-10.
He QQ, Yang L, Zhang JY, Ma JN, Ma CM. Chemical constituents of gold-red apple and their α-glucosidase inhibitory activities. J Food Sci 2014;79:C1970-83.
Amalan V, Vijayakumar N. Antihyperglycemic effect of p-coumaric acid on streptozotocin induced diabetic rats. Indian J Appl Res 2015;5:10-3.
Amalan V, Vijayakumar N, Indumathi D, Ramakrishnan A. Antidiabetic and antihyperlipidemic activity of p-coumaric acid in diabetic rats, role of pancreatic GLUT 2:In vivo
approach. Biomed Pharmacother 2016;84:230-6.
Abdel-Moneim A, Yousef AI, Abd El-Twab SM, Abdel Reheim ES, Ashour MB. Gallic acid and p-coumaric acid attenuate type 2 diabetes-induced neurodegeneration in rats. Metab Brain Dis 2017;32:1279-86.
Harini R, Pugalendi KV. Antihyperglycemic effect of protocatechuic acid on streptozotocin-diabetic rats. J Basic Clin Physiol Pharmacol 2010;21:79-91.
Semaming Y, Kukongviriyapan U, Kongyingyoes B, Thukhammee W, Pannangpetch P. Protocatechuic acid restores vascular responses in rats with chronic diabetes induced by streptozotocin. Phytother Res 2016;30:227-33.
Lin CY, Huang CS, Huang CY, Yin MC. Anticoagulatory, antiinflammatory, and antioxidative effects of protocatechuic acid in diabetic mice. J Agric Food Chem 2009;57:6661-7.
Chen HP, Liao ZP, Huang QR, He M. Sodium ferulate attenuates anoxia/reoxygenation-induced calcium overload in neonatal rat cardiomyocytes by NO/cGMP/PKG pathway. Eur J Pharmacol 2009;603:86-92.
Xiao SH. Progress in the pharmacological study and application of sodium ferulate. Traffic Med China 1998;12:484-5.
Wang BH, Ou-Yang JP. Pharmacological actions of sodium ferulate in cardiovascular system. Cardiovasc Drug Rev 2005;23:161-72.
Carpita NC. Structure and biogenesis of the cell walls of grasses. Annu Rev Plant Physiol Plant Mol Biol 1996;47:445-76.
Ursini F, Tubaro F, Rong J, Sevanian A. Optimization of nutrition: Polyphenols and vascular protection. Nutr Rev 1999;57:241-9.
Chacko SM, Nevin KG, Dhanyakrishnan R, Kumar BP. Protective effect of p
-coumaric acid against doxorubicin induced toxicity in H9c2 cardiomyoblast cell lines. Toxicol Rep 2015;2:1213-21.
Shiromwar SS, Chidrawar VR. Combined effects of p-coumaric acid and naringenin against doxorubicin-induced cardiotoxicity in rats. Pharmacognosy Res 2011;3:214-9.
Prasanna N, Rasool M. Modulation of gene-expression profiles associated with sodium arsenite-induced cardiotoxicity by p-coumaric acid, a common dietary polyphenol. J Biochem Mol Toxicol 2014;28:174-80.
Stanely Mainzen Prince P, Roy AJ. P-coumaric acid attenuates apoptosis in isoproterenol-induced myocardial infarcted rats by inhibiting oxidative stress. Int J Cardiol 2013;168:3259-66.
Semaming Y, Kumfu S, Pannangpetch P, Chattipakorn SC, Chattipakorn N. Protocatechuic acid exerts a cardioprotective effect in type 1 diabetic rats. J Endocrinol 2014;223:13-23.
Tang XL, Liu JX, Dong W, Li P, Li L, Lin CR, et al.
Cardioprotective effect of protocatechuic acid on myocardial ischemia/reperfusion injury. J Pharmacol Sci 2014;125:176-83.
Ciftci O, Disli OM, Timurkaan N. Protective effects of protocatechuic acid on TCDD-induced oxidative and histopathological damage in the heart tissue of rats. Toxicol Ind Health 2013;29:806-11.
He S, Liu F, Xu L, Yin P, Li D, Mei C, et al.
Protective effects of ferulic acid against heat stress-induced intestinal epithelial barrier dysfunction in vitro
and in vivo
. PLoS One 2016;11:e0145236.
Guslandi M. Nitric oxide: An ubiquitous actor in the gastrointestinal tract. Dig Dis 1994;12:28-36.
Badary OA, Awad AS, Sherief MA, Hamada FM.In vitro
and in vivo
effects of ferulic acid on gastrointestinal motility: Inhibition of cisplatin-induced delay in gastric emptying in rats. World J Gastroenterol 2006;12:5363-7.
Guglielmi F, Luceri C, Giovannelli L, Dolara P, Lodovici M. Effect of 4-coumaric and 3,4-dihydroxybenzoic acid on oxidative DNA damage in rat colonic mucosa. Br J Nutr 2003;89:581-7.
Barros MP, Lemos M, Maistro EL, Leite MF, Sousa JP, Bastos JK, et al.
Evaluation of antiulcer activity of the main phenolic acids found in Brazilian green propolis. J Ethnopharmacol 2008;120:372-7.
Guarner F, Malagelada JR. Gut flora in health and disease. Lancet 2003;361:512-9.
Bajaj JS, Heuman DM, Hylemon PB, Sanyal AJ, White MB, Monteith P, et al.
Altered profile of human gut microbiome is associated with cirrhosis and its complications. J Hepatol 2014;60:940-7.
Turnbaugh PJ, Ley RE, Mahowald MA, Magrini V, Mardis ER, Gordon JI, et al.
An obesity-associated gut microbiome with increased capacity for energy harvest. Nature 2006;444:1027-31.
Mathis D, Benoist C. The influence of the microbiota on type-1 diabetes: On the threshold of a leap forward in our understanding. Immunol Rev 2012;245:239-49.
Kore KJ, Bramhakule PP, Rachhadiya RM, Shete RV. Evaluation of anti ulcer activity of protocatechuic acid ethyl ester in rats. Int J Pharm Life Sci 2011;2:909.
Ma L, Wang G, Chen Z, Li Z, Yao J, Zhao H, et al.
Modulating the p66shc signaling pathway with protocatechuic acid protects the intestine from ischemia-reperfusion injury and alleviates secondary liver damage. ScientificWorldJournal 2014;2014:387640.
Tsou MF, Hung CF, Lu HF, Wu LT, Chang SH, Chang HL, et al.
Effects of caffeic acid, chlorogenic acid and ferulic acid on growth and arylamine N-acetyltransferase activity in Shigella sonnei
(group D). Microbios 2000;101:37-46.
Borges A, Ferreira C, Saavedra MJ, Simões M. Antibacterial activity and mode of action of ferulic and gallic acids against pathogenic bacteria. Microb Drug Resist 2013;19:256-65.
Shi C, Zhang X, Sun Y, Yang M, Song K, Zheng Z, et al.
Antimicrobial activity of ferulic acid against Cronobacter sakazakii
and possible mechanism of action. Foodborne Pathog Dis 2016;13:196-204.
Acar G, Dogan NM, Duru ME, Kıvrak I. Phenolic profiles, antimicrobial and antioxidant activity of the various extracts of Crocus
species in Anatolia. Afr J Microbiol Res 2010;4:1154-61.
Lou Z, Wang H, Rao S, Sun J, Ma C, Li J. p-Coumaric acid kills bacteria through dual damage mechanisms. Food Control 2012;25:550-4.
Liu WH, Hsu CC, Yin MC.In vitro
anti-helicobacter pylori activity of diallyl sulphides and protocatechuic acid. Phytother Res 2008;22:53-7.
Kuete V, Nana F, Ngameni B, Mbaveng AT, Keumedjio F, Ngadjui BT, et al.
Antimicrobial activity of the crude extract, fractions and compounds from stem bark of ficus ovata (Moraceae
). J Ethnopharmacol 2009;124:556-61.
Stojković DS, Zivković J, Soković M, Glamočlija J, Ferreira IC, Janković T, et al.
Antibacterial activity of Veronica montana
L. Extract and of protocatechuic acid incorporated in a food system. Food Chem Toxicol 2013;55:209-13.
Miklasińska M, Kępa M, Wojtyczka RD, Idzik D, Zdebik A, Orlewska K, et al.
Antibacterial activity of protocatechuic acid ethyl ester on staphylococcus aureus
clinical strains alone and in combination with antistaphylococcal drugs. Molecules 2015;20:13536-49.
Jayaraman P, Sakharkar MK, Lim CS, Tang TH, Sakharkar KR. Activity and interactions of antibiotic and phytochemical combinations against pseudomonas aeruginosa in vitro
. Int J Biol Sci 2010;6:556-68.
Kim EO, Min KJ, Kwon TK, Um BH, Moreau RA, Choi SW, et al.
Anti-inflammatory activity of hydroxycinnamic acid derivatives isolated from corn bran in lipopolysaccharide-stimulated raw 264.7 macrophages. Food Chem Toxicol 2012;50:1309-16.
Nile SH, Ko EY, Kim DH, Keum YS. Screening of ferulic acid related compounds as inhibitors of xanthine oxidase and cyclooxygenase-2 with anti-inflammatory activity. Rev Bras Farmacogn 2016;26:50-5.
Pragasam SJ, Rasool M. Dietary component p-coumaric acid suppresses monosodium urate crystal-induced inflammation in rats. Inflamm Res 2013;62:489-98.
Wei M, Chu X, Guan M, Yang X, Xie X, Liu F, et al.
Protocatechuic acid suppresses ovalbumin-induced airway inflammation in a mouse allergic asthma model. Int Immunopharmacol 2013;15:780-8.
Graf E. Antioxidant potential of ferulic acid. Free Radic Biol Med 1992;13:435-48.
Pluemsamran T, Onkoksoong T, Panich U. Caffeic acid and ferulic acid inhibit UVA-induced matrix metalloproteinase-1 through regulation of antioxidant defense system in keratinocyte HaCaT cells. Photochem Photobiol 2012;88:961-8.
Seo YK, Kim SJ, Boo YC, Baek JH, Lee SH, Koh JS, et al.
Effects of p-coumaric acid on erythema and pigmentation of human skin exposed to ultraviolet radiation. Clin Exp Dermatol 2011;36:260-6.
Hahn HJ, Kim KB, Bae S, Choi BG, An S, Ahn KJ, et al.
Pretreatment of ferulic acid protects human dermal fibroblasts against ultraviolet A irradiation. Ann Dermatol 2016;28:740-8.
Ren C, Bao YR, Meng XS, Diao YP, Kang TG. Comparison of the protective effects of ferulic acid and its drug-containing plasma on primary cultured neonatal rat cardiomyocytes with hypoxia/reoxygenation injury. Pharmacogn Mag 2013;9:202-9.
Wang B, Ouyang J, Liu Y, Yang J, Wei L, Li K, et al.
Sodium ferulate inhibits atherosclerogenesis in hyperlipidemia rabbits. J Cardiovasc Pharmacol 2004;43:549-54.
Chethan S, Dharmesh SM, Malleshi NG. Inhibition of aldose reductase from cataracted eye lenses by finger millet (Eleusine coracana
) polyphenols. Bioorg Med Chem 2008;16:10085-90.
Borate AR, Suralkar AA, Birje SS, Malusare PV, Bangale PA. Anti hyperlipidemic effect of protocatechuic acid in fructose induced hyperlipidemia in rats. Int J Pharma Bio Sci 2011;2:456-60.
Suzuki A, Kagawa D, Fujii A, Ochiai R, Tokimitsu I, Saito I, et al.
Short- and long-term effects of ferulic acid on blood pressure in spontaneously hypertensive rats. Am J Hypertens 2002;15:351-7.
Choi SW, Lee SK, Kim EO, Oh JH, Yoon KS, Parris N, et al.
Antioxidant and antimelanogenic activities of polyamine conjugates from corn bran and related hydroxycinnamic acids. J Agric Food Chem 2007;55:3920-5.
Luceri C, Giannini L, Lodovici M, Antonucci E, Abbate R, Masini E, et al.
p-Coumaric acid, a common dietary phenol, inhibits platelet activity in vitro
and in vivo
. Br J Nutr 2007;97:458-63.
[Table 1], [Table 2]