|Year : 2020 | Volume
| Issue : 1 | Page : 104-109
Antibacterial effect of combination of cinnamon essential oil and thymol, carvacrol, eugenol, or geraniol
Yassine El Atki1, Imane Aouam1, Amal Taroq1, Fatima El Kamari1, Mohammed Timinouni2, Badiaa Lyoussi1, Abdelfattah Abdellaoui1
1 Department of Biology, Laboratory of Physiology Pharmacology and Environmental Health, Faculty of Sciences Dhar El Mehraz, Sidi Mohamed Ben Abdellah University, Fez, Morocco
2 Molecular Bacteriology Laboratory, Pasteur Institute of Morocco, Casablanca, Morocco
|Date of Submission||18-Mar-2019|
|Date of Acceptance||06-Oct-2019|
|Date of Web Publication||26-Jun-2020|
Mr. Yassine El Atki
Department of Biology, Laboratory of Physiology Pharmacology and Environmental Health, Faculty of Sciences Dhar El Mehraz, Sidi Mohamed Ben Abdellah University, Fez.
Source of Support: None, Conflict of Interest: None
Bacterial resistance to classic antibiotics is an alarming rate to put this into control with the use of natural products of plant derivatives. The objective of this study was to determine the phytochemical of cinnamon essential oil (EO) and to evaluate its antibacterial activity alone and in combination with some main components of EOs such as thymol, carvacrol, eugenol, or geraniol against three bacterial strains (Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa). The phytochemical analysis of cinnamon EO was evaluated using gas chromatography-flame ionization detector and gas chromatography-mass spectrometer analysis. The antibacterial activity of tested compounds was determined by agar disk diffusion and minimum inhibitory concentration (MIC) assays. The checkerboard method was used to quantify the efficacy of cinnamon EO in combination with those compounds. The results showed that the major compound in the cinnamon EO was trans-cinnamaldehyde (91.01%). Cinnamon oil was the highest antibacterial activity with MIC of 0.005, 0.005, and 0.02 mg/mL against E. coli, S. aureus, and P. aeruginosa, respectively. Synergistic activity was shown only against S. aureus by the combination of cinnamon EO and thymol. The additive effect was found against E. coli when cinnamon EO was combined with thymol or carvacrol, and against S. aureus when cinnamon EO was combined with carvacrol. However, the combination of EO and thymol or carvacrol showed an indifference action against P. aeruginosa. The combination of cinnamon EO with thymol or carvacrol can be used as an alternative therapeutic agent for medical application and as a natural preservative.
Keywords: Antibacterial activity, cinnamon, combination, gas chromatography-mass spectrometer analysis
|How to cite this article:|
El Atki Y, Aouam I, Taroq A, El Kamari F, Timinouni M, Lyoussi B, Abdellaoui A. Antibacterial effect of combination of cinnamon essential oil and thymol, carvacrol, eugenol, or geraniol. J Rep Pharma Sci 2020;9:104-9
|How to cite this URL:|
El Atki Y, Aouam I, Taroq A, El Kamari F, Timinouni M, Lyoussi B, Abdellaoui A. Antibacterial effect of combination of cinnamon essential oil and thymol, carvacrol, eugenol, or geraniol. J Rep Pharma Sci [serial online] 2020 [cited 2020 Jul 13];9:104-9. Available from: http://www.jrpsjournal.com/text.asp?2020/9/1/104/287584
| Introduction|| |
Antibacterial resistance is widely known as a dangerous level in all parts of the world. Overuse and misuse of antibiotics in clinical context are extensively considered as major pathways of promoting antibiotic resistance. Other nonclinical large-scale uses of antibiotics in aquaculture, livestock, and poultry farms have strongly contributed to antibiotic contaminations with development of pathogenic bacterial resistance. The discovery of new antibacterial agents is mainly based on natural products that can be obtained from different sources, including plants, animals, algae, fungi, and bacteria, but there has been a growing interest in bioactive compounds provided by the plant as an alternative to the common antibiotic. Essential oils (EOs) account for a source of very promising natural compounds for producing new antibacterial drugs. Numerous studies have reported a strong antibacterial effect of EOs.,,, Among these oils, the potential antibacterial of cinnamon has been documented frequently.,,,
Furthermore, the combinations, either single EO or mixtures of purified main components (MCs), would assure the exhibition of the target bacteria to many chemical compounds and usually lead to better activity. Combining different EO has been recently studied in a view to increasing theirs antibacterial effects without increasing theirs concentrations.,, It was argued that the combined treatment with cinnamon and some plants EOs showed an additive effect against bacterial species as compared with their pure EOs., The combination of cinnamonmustard EOs possesses an additive effect against some food-borne bacteria. In another study, cinnamon oil combined with thyme or clove displayed an additive antibacterial effect. An important synergistic effect of some antibiotics and cinnamon against Staphylococcus aureus and Escherichia coli was proved. On the contrary, the antibacterial propriety of the MCs of EOs, including thymol, carvacrol, and eugenol, largely studied, and it has been proved to be a strong antimicrobial.,, Although the antibacterial mechanism of EOs and their constituents is not fully understood, recent studies have shown that constituents with a phenolic structure, such as thymol carvacrol and eugenol, have the greatest bactericidal activities, followed by aldehydes, ketones, alcohols, ethers, and hydrocarbons.,, However, to the best of our knowledge, there are no available data about the antibacterial effect of cinnamon EO combined with the main monoterpenes of EOs such as thymol, carvacrol, eugenol, and geraniol, which are frequently absent in cinnamon EO. The aim of this study was to search the possible synergistic antibacterial effect of cinnamon EO associated with certain compounds such as thymol, carvacrol, eugenol, or geraniol against E. coli ATCC 25922, S. aureus ATCC 25923, and Pseudomonas aeruginosa ATCC 27853.
| Materials and Methods|| |
Essential oil extraction
The barks of Cinnamomum cassia (cinnamon) were purchased from a local supermarket in Fez (Morocco). Identification was confirmed by professor Amina Bari, a botanist at the Department of Biological Sciences, Sidi Mohammed Ben Abdellah University, Morocco. A powder of 200 g cinnamon were hydro-distillated for 3h with 700 mL of water using a modified Clevenger-type apparatus: the hydrosol was collected in a separating flask, so that the heavy oil was decanted at the bottom of the flask, whereas the water of the hydrosol was recycled into the flask containing the boiling powder plant. The obtained EO was stored at 4°C before analysis.
Chemical analysis of the essential oils
Gas chromatography-flame ionization detector
The cinnamon EO was diluted in hexane and 1 μL of diluted EO was sampled for the gas chromatographic analysis. Trace gas chromatograph (GC) (ULTRA S/N 20062969, Thermo Fischer, Waltham, USA), the GC (TRACE GC-ULTRA, S/N 20062969, Thermo-Fischer) analysis equipped with flame ionisation detector (GC-FID), using an HP-5MS nonpolar fused silica capillary column (60 m × 0.32 mm, film thickness 0.25 μm). The operating conditions were as follows: oven temperature program from 50 °C (2 min) to 280 °C at 5 °C/min and the final temperature kept for 10 min; “split mode” ratio 1:20; carrier gas nitrogen, flow rate 1 mL/min; temperature of detector (flame ionization detector) and injector were fixed at 280 °C and 250 °C, respectively.
Gas chromatography-mass spectrometry analysis
The cinnamon EO was analyzed by a capillary GC (Thermo Fischer) directly coupled to the mass spectrometer system (model GC ULTRA S/N 20062969; Polaris QS/N 2107, Thermo-Fischer; Waltham, USA), involving an HP-5MS apolar fused silica capillary column (60 m × 0.32mm, 0.25 µm ﬁlm thickness). The operating condition of GC-MS oven temperature was as follows: initial temperature 40°C for 2 min, programmed rate 2°C per min up to final temperature 260°C with isotherm for 10 min; injector temperature 250°C. The carrier gas was the helium with a flow rate of 1 mL/min. The EO sample was diluted in hexane. The injected specimen volume was 1 µL of diluted EO; systems were operated with a split ratio of 1:15. Ionisation of the sample components was performed in electron impact mode (EI, 70 eV). The ion source temperature was fixed to 200°C. The mass range from 40–650 amu, was scanned at a rate of 2.9 scans/s and Transfer line temperature was 300°C. The characterization of the components was determined by their retention indices (RI) relative to those homologous n-alkanes (C8–C20) series (Fluka, Buchs/sg, Switzerland) and by matching their recorded mass spectra with those stored in the database of spectrometer (NIST MS Library v. 2.0) and the bibliography.
Bacterial strains and inoculums standardization
In this study, the antibacterial activity of cinnamon oil, alone and in combination with some MCs of EOs such as thymol, carvacrol, eugenol, or geraniol (Sigma-Aldrich, St. Louis, MO), was tested against three bacterial strains: E. coli ATCC 25922, S. aureus ATCC 25923, and P. aeruginosa ATCC 27853, which was provided by the Pasteur Institute of Casablanca (Morocco). The inoculum suspension was obtained by taking colonies from 24-h cultures. The colonies were suspended in sterile 0.9% aqueous solution of NaCl. The density was adjusted to the turbidity of a 0.5 McFarland standard (1–5 × 108 CFU/mL).,
Agar disk-diffusion assay
The agar disk-diffusion assay was determined in triplicate according to the Kirby–Bauer experiment ; the suspensions of microorganisms (1–5 108 CFU/mL) were flood inoculated on to the surface of Mueller–Hinton (MH) agar plates. Sterile filter disks of 6mm diameter (Whatman Paper No. 3) were impregnated with 10 μg/disk of the compound and were put on to the surface of the inoculated MH agar. All plates were incubated for 18h at 37°C. Antibacterial effect was evaluated by measuring the inhibition zones.
Determination of the minimum inhibitory concentration
The minimum inhibitory concentration (MIC) was performed using a microdilution assay in 96-well microtiter plates according to the National Committee for Clinical Laboratory Standards (NCCLS). In fact, different concentrations of cinnamon EO and MCs were prepared in a suspension containing 0.2% agar in sterile distillated water in order to disperse the compounds without adding solvent or detergent. They were carried out by successive dilutions 1/2 ranging from 1.25 to 0.002 mg/mL for EO, thymol, and carvacrol and from 25 to 0.04 mg/mL for eugenol and geraniol. Bacterial suspensions were prepared in the same manner described previously and plated in 96-well plates at a density of 1–5× 106 CFU/mL. Cinnamon EO or MCs were added at different concentrations at the corresponding wells in microtiter plates. Finally, all plates were incubated during 18h at 37°C; bacterial proliferation was visually by adding to each well 20 µL of 2, 3, 5-triphenyltetrazolium chloride (TTC) aqueous solution (1%), with additional incubation for 1h. MIC was the lowest concentration that does not produce red color.
The evaluation of the interaction between cinnamon EO and MCs (thymol, carvacrol, eugenol, or geraniol) was carried out according to the method of Moody. Briefly, ten concentrations of cinnamon EO and eight concentrations of the MCs were prepared in sterile tubes by dilutions 1/2. EO at decreasing concentrations, going from MIC × 4 to MIC/128, was introduced horizontally into 96-well microtiter plates. In the same manner, the MCs at decreasing concentrations, going from MIC × 4 to MIC/32, were introduced vertically. The final volume in each well was 200 μL comprising 25 μL of EO, 25 µL of MC dilution, and 150 μL of MH media containing 1–5×106 CFU/mL of bacterial suspensions. All plates were then incubated during18h at 37 °C. The analysis of the combination was obtained by calculating the fraction inhibitory concentration index (FICI) using the following formula:
where (A) is cinnamon EO and (B) is one of the MCs.
The FICI values were interpreted as follows: a synergistic effect when FICI ≤ 0.5; an additive effect when 0.5 ˂ FICI ˂1; an indifferent (no interaction) when 1˂ FICI ˂4; and an antagonistic effect when FICI > 4.
| Results|| |
Essential oil composition
The yield of the EO of cinnamon was 1.26% (v/w, dark yellow)calculated on a dry weight basis. The GC–MS analysis of EO is presented in [Table 1]. Eleven components were identified comprising 98.44% of the total amount. Trans-cinnamaldehyde was found as the single major component (91.01%). The other component’s insignificant percent were as follows: cis-cinnamyle acetate (2.04%), linalool (1.3%), caryophyllene oxide (1.06%), γ-terpinene (1.03%), and δ-cadinene (0.9%)[Table 1].
The antibacterial activity of the cinnamon EO, thymol, carvacrol, eugenol, and geraniol, tested against three bacterial strains (E. coli ATCC 25922, S. aureus ATCC 25923, and P. aeruginosa ATCC 27853), is shown in [Table 2]. Cinnamon EO was the strongest antibacterial effect against all strains tested: E. coli, S. aureus, and P. aeruginosa, with the MIC values of 0.005, 0.005, and 0.02 mg/mL, respectively. In addition, E. coli and S. aureus were sensitive to the thymol and carvacrol (MIC: 0.072 and 0.31 mg/mL, respectively) and were resistant to eugenol and geraniol. Pseudomonas aeruginosa was relatively resistant to all MCs tested.
|Table 2: Antibacterial activity of cinnamon EO, thymol, carvacrol, eugenol, and geraniol|
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Combined effects of cinnamon essential oil and main components
Synergistic interactions between different plant extracts are the aim of the herbal formulation in folk medicine. The interaction of plant with MCs of EOs is one of the novel ways to inhibit the resistance mechanisms of bacteria. In this study, the combined antibacterial effect of cinnamon oil was evaluated by the checkerboard method, in order to determine the fractional inhibitory concentration (FIC). [Table 3] shows the cinnamon EO tested against three bacterial strains in combination with some MCs. FICI were calculated and interpreted as synergy, addition, indifference, or antagonism. As shown in [Table 3], mixing MCs with cinnamon EO reduces the MICs, 2-fold for P. aeruginosa and 2–8-fold for E. coli and S. aureus. The synergistic activity was obtained only by the combination of cinnamon EO and thymol against S. aureus, with the FICI value of 0.5. In addition, the combination of cinnamon oil with thymol showed an additive effect against E. coli with the FICI of 0.75. An additive effect was also found when cinnamon oil was combined with carvacrol against E. coli and S. aureus with the FICI of 1 and 0.625, respectively, whereas the combination of cinnamon with thymol or carvacrol showed an indifferent effect against P. aeruginosa. However, the combination of cinnamon EO and eugenol or geraniol displayed an antagonism action against all bacteria tested [Table 3].
| Discussion|| |
Eleven components were identified in EO of cinnamon bark. Trans-cinnamaldehyde was found as its single major component (91.01%). Several studies on the chemical composition of cinnamon EO were reported and showed that trans-cinnamaldehyde was the single major compound.,, Simic et al. reported trans-cinnamaldehyde (62.8%) and cinnamaldehyde propylene (5.5%) as the major compounds from cinnamon (C. zeylanicum) volatile oil. Marongino et al. found that the MCs of cinnamon (C. zeylanicum) were trans-cinnamaldehyde (77.1%), trans-β-caryophyllene (6.0%), and α-terpineol (4.4%).
Concerning the antibacterial effect, our results are higher than that described by Clemente et al., who found that cinnamon EO inhibits the growth of E. coli, S. aureus, and P. aeruginosaat MIC values of 0.4, 0.4, and 0.2 mg/mL, respectively. They are in accordance with the data reported by Gallucci et al.Escherichia coli and S. aureus are sensitive to thymol and carvacrol (MIC: 15.07, 7.53,7.62, and 3.82 mg/mL, respectively), and resistant to eugenol and geraniol (MIC > 20 mg/mL). Pei et al. revealed that E. coli growth was inhibited by thymol, carvacrol, and eugenol at MIC values of 1.6, 0.4, and 0.4 mg/mL, respectively. In a study conducted by Miladi et al., carvacrol had better activity against S. aureus (MIC: 64 mg/mL) and thanthymol and eugenol at MIC values of 256 mg/mL. In addition, the sensitivity of P. aeruginosa to cinnamon EO is probably attributed to the combined effects of several compounds constituting this EO, acting on various cell targets. Bouhdid et al. showed that cinnamon EO damages the cell membrane of P. aeruginosa, which leads to cell death. According to the previous studies, the high antibacterial activity observed with cinnamon EO may be because of the action of trans-cinnamaldehyde, which is considered as its single major compound.,, It has been reported that trans-cinnamaldehyde possess the highest antimicrobial activity in comparison with other constituents of cinnamon oil.,, Furthermore, thymol, carvacrol, eugenol, and geraniol are the main compounds of thyme, oreganum, clove, and geranium plants, respectively. The antibacterial effect of their EOs has been reported., Moreover, several authors show the antibacterial activity of various EOs plants including, thyme, oregano, lemon balm, basil, marjoram, and baccharis against the bacterial strains under consideration in this study.,
Previous studies have explored the antibacterial effect of cinnamon EO combinations. Clemente et al. demonstrated that the combination of cinnamon with mustard EOs showed an additive effect against E. coli ATCC 25922, P. aeruginosa ATCC 27853, and some other bacteria species. Lu et al. used the checkerboard assay to determine the activity of cinnamon combined with thyme and clove EOs. Both cinnamon combinations displayed in most cases an additive or indifferent action against food-borne bacteria. These results were confirmed by this study, thus highlighting the effectiveness of cinnamon oil when combined with thymol or carvacrol. Moreover, eugenol and geraniol are the MCs of EOs from clove and pelargonium, respectively. The additive effects of these EOs have been reported.,
On the contrary, the mechanism that is responsible for the antimicrobial activity of cinnamon includes its chemical composition such as cinnamaldehyde, which is an electronegative molecule that could interfere with the biological process in microorganism particularly nitrogen containing substances such as proteins and nucleic acids. Furthermore, cinnamon EOs and their MCs have been reported to inhibit bacteria via Antiquorum sensing effects, inhibiting cell division, ATPase, biofilm formation membrane porine, and mobility; altering the lipid profile; and thereby acting cell membrane producing lumps and autoaggregation. Furthermore, thymol and carvacrol are phenolic compounds; their hydroxyl groups play a major role in their antibacterial activities. They are able to alter the cell outer membrane and combine with the charged groups of membrane through increasing its permeability. Furthermore, carvacrol had ATPase inhibitory propriety, which causes dissipation of the motive force of the proton, and can subsequently inhibit other enzymes. However, there are limited reports on the action mechanisms of a mixture of EOs and theirs purified components on bacteria. Nevertheless, it is possible to explain the synergistic or additive effects caused by the combination of cinnamon EO and thymol or carvacrol by the fact that the thymol or carvacrol could increase the cell membrane permeability, making it easier for cinnamon compounds to penetrate into the cell and combine with proteins and nucleic acids. In addition, some explanations for the mechanisms of antibacterial interaction that produce antagonism include the use of compounds that act on the same target of the microorganism, combinations of bactericidal and bacteriostatic agents, and chemical interactions between compounds.,
| Conclusion|| |
This work has shown that cinnamon EO possesses a stronger antimicrobial effect than all main compounds tested against resistant bacteria. This effect could be because of the trans-cinnamaldehyde, which is considered as the major compound of this EO. The synergistic effect was shown only against S. aureus with the combination of cinnamon oil and thymol. In addition, we have shown that the combination of cinnamon EO with thymol or carvacrol and their synergistic or additive effects can be used as an alternative therapeutic agent for medical application, as a natural preservative and food additive.
The authors would like to thank the Pasteur Institute of Casablanca, Morocco, for providing multidrug-resistant bacteria, and PhD. Soumia Ait Assou (Laboratory of Biotechnology, Faculty of Sciences Dhar El Mehraz, fez, Morocco) for his critical reading of the manuscript.
Financial support and sponsorship
This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.
Conflicts of interest
There are no conflicts of interest.
| References|| |
Martelli G, Giacomini D. Antibacterial and antioxidant activities for natural and synthetic dual-active compounds. Eur J Med Chem 2018;158:91-105.
Kaur SP, Rao R, Nanda S. Amoxicillin: A broad spectrum antibiotic. Int J Pharm Pharm Sci 2011;3:30-7.
Rossiter SE, Fletcher MH, Wuest WM. Natural products as platforms to overcome antibiotic resistance. Chem Rev 2017;117:12415-74.
El Atki Y, Aouam I, El kamari F, Taroq A, Nayme K, Timinouni, et al
. Antibacterial activity of cinnamon essential oils and their synergistic potential with antibiotics. J Adv Pharm Technol Res 2019;10:63-7.
Taroq A, El Kamari F, Oumokhtar B, Aouam I, El Atki Y, Lyoussi B, et al
. Phytochemical screening of the essential oil of syzygium aromaticum and antibacterial activity against nosocomial infections in neonatal intensive care. Int J Pharm Sci Rev Res 2018;48:58-61.
El Kamari F, Taroq A, El Atki Y, Aouam I, Lyoussi B, Abdellaoui A. Chemical composition of essential oils from vitex agnus-castus l. Growing in Morocco and its in vitro
antibacterial activity against clinical bacteria responsible for nosocomialinfections. Asian J Pharm Clin Res 2018;7:365-8.
Fathollahi R, Dastan D, Lari J, Masoudi S. Chemical composition, antimicrobial and antioxidant activities of Crupina crupinastrumas a medicinal plant growing wild in West of Iran. J Rep Pharma Sci 7;2018:174-82.
Vasconcelos NG, Croda J, Simionatto S. Antibacterial mechanisms of cinnamon and its constituents. Microb Path 2018;120:198-203.
Unlu M, Ergene E, Unlu GV, Zeytinoglu HS, Vural N. Composition, antimicrobial activity and in vitro cytotoxicity of essential oil from Cinnamomum zeylanicum
blume (lauraceae). Food Chem Toxicol 2010;48:3274-80.
Singh G, Maurya S, DeLampasona MP, Catalan CA. A comparison of chemical, antioxidant and antimicrobial studies of cinnamon leaf and bark volatile oils, oleoresins and their constituents. Food Chem Toxicol 2007;45:1650-61.
Firmino DF, Cavalcante TTA, Gomes GA, Firmino NCS, Rosa LD, De Carvalho MG, et al
. Antibacterial and antibiofilm activities of Cinnamomum Sp
. essential oil and cinnamaldehyde: Antimicrobial activities. Sci W J 2018;2018:1-9.
Shi C, Zhang X, Zhao X, Meng R, Liu Z, Chen X, et al
. Synergistic interactions of nisin in combination with cinnamaldehyde against Staphylococcus aureus
in pasteurized milk. Food Contr 2017;71:10-16.
Gutierrez J, Barry-Ryan C, Bourke P. The antimicrobial efficacy of plant essential oil combinations and interactions with food ingredients. Int J Food Microbiol 2008;124:91-7.
Al-Bayati FA. Synergistic antibacterial activity between thymus vulgaris and Pimpinella anisum
essential oils and methanol extracts. J Ethnopharmacol 2008;116:403-6.
Clemente I, Aznar M, Silva F, Nerín C. Antimicrobial properties and mode of action of mustard and cinnamon essential oils and their combination against foodborne bacteria. Innov Food Sci Emerging Technol 2016;36:26-33.
Lu F, Ding YC, Ye XQ, Ding YT. Antibacterial effect of cinnamon oil combined with thyme or clove oil. Agr Sci China 2011;10: 1482-7.
El Atki Y, Aouam I, El kamari F, Taroq A, Lyoussi B, Oumokhtar B, et al
. Phytochemistry, antioxidant and antibacterial activities of two Moroccan Teucrium polium L
. subspecies: Preventive approach against nosocomial infections. Arabian J Chem 2019;12.
Miladi H, Zmantar T, Kouidhi B, Chaabouni Y, Mahdouani K, Bakhrouf A, et al
. Use of carvacrol, thymol, and eugenol for biofilm eradication and resistance modifying susceptibility of salmonella enterica serovar typhimurium strains to nalidixic acid. Microb Pathog 2017;104:56-63.
Netopilova M, Houdkova M, Rondevaldova J, Kmet V, Kokoska L. Evaluation of in vitro growth-inhibitory effect of carvacrol and thymol combination against Staphylococcus aureus
in liquid and vapour phase using new broth volatilization chequerboard method. Fitoterapia 2018;129:185-90.
El Atki Y, Aouam I, El kamari F, Taroq A, Gourch A, Abdellaoui A, et al
. Antibacterial efficacy of thymol, carvacrol, eugenol and menthol as alternative agents to control the growth of nosocomial infection-bacteria. J Pharm Sci Res 2019;11:306-9.
Adams RP. Identification of Essential Oil Components by Gas Chromatography/Massspectrometry. Carol Stream, IL:Allured Publishing Corporation;2007. p. 456.
Mello S, Bittencourt F, Fronza N, Cunha A, Neud G, Rosana C, et al
. Chemical composition and antibacterial activity of Laurus nobilis
essential oil towards foodborne pathogens and its application in fresh Tuscan sausage stored at 7 C. LWT – Food Sci Technol 2014;59:86-93.
Gary L, Furtado L. Single-disk diffusion testing (Kirby–Bauer) of susceptibility of proteus mirabilis to chloramphenicol: Significance of the intermediate category. J Clinl Microbiol 1980;14:550-3.
NCCLS (National Committee for Clinical Laboratory Standard). Performance Standards for Antimicrobial Susceptibility Testing, Ninth International Supplement. Delaware County, PA: NCCLS;1999.
Remmal A, Bouchikhi T, Rhayour K. Improved method for the determination of antimicrobial activity of essential oils in agar medium. J Essent Oil Res 1993;5:179-84.
Moody JA. Synergism testing: Broth microdilution checkerboard and broth microdilution. In: Isenberg H.D., editor, Clinical Microbiology Procedures Handbook. Washington, DC: American Society for Microbiology; 2003. p. 1-28.
Fadli M, Saad A, Sayadi S, Chevalier J, Mezrioui NE, Pagès JM, et al
. Antibacterial activity of thymus maroccanus and thymus broussonetii essential oils against nosocomial infection: Bacteria and their synergistic potential with antibiotics. Phytomedicine 2012;19:464-71.
Simić A, Soković MD, Ristić M, Grujić-Jovanović S, Vukojević J, Marin PD. The chemical composition of some lauraceae essential oils and their antifungal activities. Phytother Res 2004;18:713-7.
Marongiu B, Piras A, Porcedda S, Tuveri E, Sanjust E, Meli M, et al
. Supercritical CO2 extract of Cinnamomum zeylanicum
: Chemical characterization and antityrosinase activity. J Agric Food Chem 2007;55:10022-7.
Gallucci MN, Oliva M, Casero C, Dambolena J, Luna A, Zygadlo J, et al
. Antimicrobial combined action of terpenes against the food‐borne microorganisms Escherichia coli, Staphylococcus aureus
and Bacillus cereus
. Flav Frag J 2009;24:348-54.
Pei RS, Zhou F, Ji BP, Xu J. Evaluation of combined antibacterial effects of eugenol, cinnamaldehyde, thymol, and carvacrol against E. Coli
with an improved method. J Food Sci 2009;74:M379-83.
Miladi H, Zmantar T, Kouidhi B, Al Qurashi YMA, Bakhrouf A, Chaabouni Y, et al
. Synergistic effect of eugenol, carvacrol, thymol, p-cymene and γ-terpinene on inhibition of drug resistance and biofilm formation of oral bacteria. Microb Pathog 2017;112:156-63.
Bouhdid S, Abrini J, Amensour M, Zhiri A, Espuny MJ, Manresa A. Functional and ultrastructural changes in Pseudomonas aeruginosa
and Staphylococcus aureus
cells induced by Cinnamomum verum
essential oil. J Appl Microbiol 2010;109:1139-49.
Cheng SS, Liu JY, Hsui YR, Chang ST. Chemical polymorphism and antifungal activity of essential oils from leaves of different provenances of indigenous cinnamon (Cinnamomum osmophloeum
). Bioresour Technol 2006;97:306-12.
Ouedrhiri W, Balouiri M, Bouhdid S, Moja S, Chahdi FO, Taleb M, et al
. Mixture design of Origanum compactum, Origanum majorana
and Thymus serpyllum
essential oils: Optimization of their antibacterial effect. Ind Crops Prod 2016;89:1-9.
Boukhatem MN, Kameli A, Saidi F. Essential oil of Algerian rose-scented geranium (Pelargonium graveolens
): Chemical composition and antimicrobial activity against food spoilage pathogens. Food Contr 2013;34:208-13.
Rosato A, Vitali C, De Laurentis N, Armenise D, Antonietta Milillo M. Antibacterial effect of some essential oils administered alone or in combination with norfloxacin. Phytomedicine 2007;14:727-32.
Wendakoon CN, Sakaguchi M. Inhibition of amino acid decarboxylase activity of enterobacter aerogenes by active components in spices. J Food Prot 1995;58:280-3.
Saad NY, Muller CD, Lobstein A. Major bioactivities and mechanism of action of essential oils and their components. Flav Frag J 2013;28:269-79.
Burt S. Essential oils: Their antibacterial properties and potential applications in foods. Int J Food Microbiol 2004;94:223-53.
Cristani M, D’Arrigo M, Mandalari G, Castelli F, Sarpietro MG, Micieli D, et al
. Interaction of four monoterpenes contained in essential oils with model membranes: Implications for their antibacterial activity. J Agric Food Chem 2007;55:6300-8.
Gill AO, Holley RA. Inhibition of membrane bound ATPases of Escherichia coli
and Listeria monocytogenes
by plant oil aromatics. Int J Food Microbiol 2006;111:170-4.
Goñi P, López P, Sánchez C. Antimicrobial activity in the vapour phase of a combination of cinnamon and clove essential oils. Food Chem 2009;116:982-9.
Cox S, Mann C, Markham J. Interactions between components of the essential oil of Melaleuca alternifolia
.J Appl Microbiol 2001;91:492-7.
[Table 1], [Table 2], [Table 3]