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   Table of Contents - Current issue
Coverpage
January-June 2018
Volume 7 | Issue 1
Page Nos. 1-78

Online since Tuesday, January 9, 2018

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RESEARCH ARTICLES  

Therapeutic effect of Ferulago angulata extract on reproductive parameters and serum testosterone levels in diabetic male rats p. 1
Glavizh Rostami Nassab, Somayeh Bohlouli, Ali Ghanbari
Diabetes is an important metabolic disease inducing different effects on body organs, especially reproductive system. Increased oxidative stress and antioxidant capacity changed in diabetes induce infertility and decrease the sperm parameters. This study was to evaluate the therapeutic effects of hydro alcoholic extract of Ferulago angulata on reproductive parameters in diabetic male rats. In this experimental study, we used 30 male Wistar rats (230- 250g) with an average age of 10 weeks. A total of 24 rats were made diabetes type I by 40 mg/kg streptozotosin. Animals were divided into 5 groups of control, diabetic, and diabetic+ Ferulago angulata extract (100, 200 and 400mg/kg). Sperm parameters, serum testosterone level, seminiferous tubules diameter, and germ line epithelium maturity were assayed at the end of study. Data were analyzed by one-way ANOVA test and P<0.05 was considered statistically significant. Our results showed serum testosterone level and sperm parameters, including count, viability, progressive motility, and normal morphology as well as seminiferous tubules diameter and germ line epithelium maturity of diabetic male rats increased at 200 and 400 mg/kg doses of Ferulago angulata extract (P<0.05). The hydroalcoholic extract of Ferulago angulata, an herbal plant with abundant antioxidants, improved the quality of sperm and reproductive parameters in diabetic male rats.
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Two phase solvent extraction method for analysis of methadon in immature human milk, a breast feeding recommendation in early postpartum period p. 9
Nasrin Jalilian, Farhad Ahmadi, Elham Farhadi
In this work we introduced a new two phase freezing (TPF) method coupled with gas chromatography for the extraction, clean up and determination of methadone (MT) in human milk samples. TPF procedure was optimized for extraction of MT from immature milk sample. The extraction of MT was performed from 1.0 ml of milk that contain 0.2 ml of Briton Robinson buffer (pH=2.5) and 0.3 ml of acetonitrile. For separation of acetonitrile from aqueous solution, the solution was placed in refrigerator at −40 °C. The MT was analyzed by gas chromatography. The results demonstrated that the amount of MT that transferred to milk is significantly different from other published reports. The immature milks of six women who were used MT (dose of 90 mg/day) in duration of 1, 2, 3, and 5 h after consumption were analyzed. Our data demonstrated that, before one hour and also 5 h after MT consumption the breastfeeding is safe and between 2-4 h after consumption dose not safe neither.
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SHORT COMMUNICATION Top

Apoptosis cell death effect of linoleic acid from nigella sativa on human ovary cancer cells through mitochondrial intrinsic pathway p. 20
Yalda Shokoohinia, Gholamreza Bahrami, Fatemeh Taherabadi, Fataneh Jaffari, Leila Hosseinzadeh
In this study, we evaluated the cytotoxic potential of fractions (F1-F5) isolated from hexane extract of the seeds of N. sativa on human ovarian carcinoma cell line, A2780. F2 showed an outstanding potent cytotoxic effect against A2780 cells. Next, this fraction was purified to obtain six sub-fractions (SF1-SF6) and their cytotoxic effects were then evaluated. The obtained results showed that SF2 had strong cytotoxic effect against A2780 cell line. The effective sub-fraction (SF2) was determined to be linoleic acid (LA) according to spectroscopic analyses. In the next set of experiments, the apoptotic potentials of LA were investigated. Induction of apoptosis by LA was accompanied by an increase in activation of caspase-3, -9 and reduction in mitochondrial membrane potential (MMP) in A2780 cells. It can be concluded that LA, inhibited the growth of human ovarian carcinoma cells, A2780 and induced mitochondrial-related apoptosis.
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RESEARCH ARTICLES Top

Gas chromatographic analysis of sodium valproate in plasma and urine after air assisted liquid-liquid microextraction p. 27
Maryam Abbaspour, Mir Ali Farajzadeh, Maryam Khoubnasabjafari, Sajad Haririan, Abolghasem Jouyban
Rapid, highly efficient, and reliable liquid–liquid microextraction (LLME) methods followed by gas chromatography-flame ionization detection were developed for the extraction, preconcentration, and determination of valproate in human plasma and urine samples. Proteins of plasma sample are precipitated by adding methanol and urine sample was diluted prior to performing the microextraction procedures. Fine organic solvent droplets were formed by repeated suction and injection of the mixture of sample solution and extraction solvent into a test tube with a glass syringe. After extraction, phase separation was performed by centrifuging and the enriched analytes in the sedimented organic phase were determined by the separation system. The main factors influencing the extraction efficiency including extraction solvent type and volume, salt addition, pH, and extraction times are investigated. Under the optimized conditions, the proposed method showed good precision (relative standard deviation less than 8%). Limits of detection and lower limits of quantification for valproate were obtained in the ranges of 0.05.0.22 and 0.1.0.5μg mL-1, respectively. The linear ranges were 0.5–500 and 0.1–200 μg mL-1in plasma and urine, respectively (r2 ≥ 0.9995). The relative recoveries varied from 98–102 % and 93-100 %, respectively for plasma and urine samples. The mean relative standard deviations for intra-assay and inter-assay precisions were 3.4 % and 6.0 %, respectively. Preconcentration factors were in the range of 7-44. Good recoveries (55–86%) were obtained for the spiked samples. The proposed method was successfully used to analyze plasma and urine samples of epileptic receiving sodium valproate.
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Synthesis of novel amide containing schiffs bases of 5-(4-chloro-phenyl)-furan-2-carboxaldehyde: Their In Vivo anti-inflammatory, antioxidant and antinociceptive activities with ulcerogenic risk evaluation p. 44
Saqlain Haider, Mohammad Sarwar Alam, Hinna Hamid, Sadiq Umar, Deepak Kumar, Syed Nazreen
A library of eighteen amide containing Schiffs bases has been synthesized and screened for their anti-inflammatory, antioxidant and antinociceptive activities. The compound 2 (COX-1 IC50 = 63.23 μM; COX-2 IC50 = 1.80 μM; SI = 35.12) exhibited potent selective COX-2 inhibition as compared to indomethacin (COX-1 IC50 = 3.60 μM; COX-2 IC50 = 7.50 μM; SI = 0.48). The compounds 2 and 7 reduced the COX-2 level to 7.5 ±0.35 nmole/min/ml and 6.8 ± 0.32nmole/min/ml respectively. The compounds 6 exhibited reduced the TNF-α level to 3.36 ± 0.18pg/ml. The compounds 2, 6, 7, 13 and 17 did not induce any gastric ulceration in comparison to the standard drug indomethacin.
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Optimization of lipofectamine-2000/siRNALipoplexLoaded PLGANanoparticles for efficient EGFR gene silencing: An in Vitro study p. 64
Ali Fattahi, Behzad Shahbazi, Leila Hosseinzadeh, Ghobad Mohammadi
In this study, a novel small interfering RNA (siRNA) delivery system based on encapsulation of lipolexes was introduced. A Lipofectamine-2000–siRNA complex was encapsulated in particles of poly (D,L-lactic-co-glycolic acid; PLGA) by double micro-emulsion. Parameters such as surfactant concentration, the volume of the inner water phase and the outer water phase were evaluated to achieve high loading efficiency, small particle size and low polydispersity. The ratio of the internal to the external phase has a significant effect on the particle size and encapsulation efficiency. The various concentration of surfactant has a different effect on the particle size. In order to achieve optimum conditions for siRNA delivery, the luciferase siRNA was used as a reporter gene. The prepared formulations have a particle sizes in the range of 222 ± 5.2 nm to 900 ± 20 nm and loading efficiency in the range of 4% to 29%. lipoplex loaded PLGA particles (LPPs) had a zeta potential values ranging from −23±2.5 to −29±1.5 mV. S1 and S3 formulations showed greater efficiency compared to the lipoplexes. The gene silencing pattern of LPPs was different from lipoplex. The cytotoxicity of lipoplex loaded PLGA particles (LPPs) was lower than lipoplexes in H1299 cell line. LPPs showed better stability and higher level transfection in the presence of heparin than lipoplexes. The EGFR silencing of S1 formulation was greater than other formulation in A431 cell line. All together these properties suggest that lipoplex loaded PLGA particles have strong potential as a gene carrier for in vivo silencing angiogenesis and treatment of cancer.
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