Journal of Reports in Pharmaceutical Sciences

RESEARCH ARTICLE
Year
: 2018  |  Volume : 7  |  Issue : 1  |  Page : 9--19

Two phase solvent extraction method for analysis of methadon in immature human milk, a breast feeding recommendation in early postpartum period


Nasrin Jalilian1, Farhad Ahmadi2, Elham Farhadi1 
1 Department of Obstetrics and Gynecology, Maternity Research Center, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran
2 Department of Medicinal Chemistry, School of Pharmacy-International Campus, University of Medical Sciences, Tehran; Nano Drug Delivery Research Center, Kermanshah University of Medical Science, Kermanshah, Iran

Correspondence Address:
Farhad Ahmadi
Department of Medicinal Chemistry, School of Pharmacy-International Campus, Iran University of Medical Sciences, Tehran; Nano Drug Delivery Research Center, Kermanshah University of Medical Science, Kermanshah
Iran

In this work we introduced a new two phase freezing (TPF) method coupled with gas chromatography for the extraction, clean up and determination of methadone (MT) in human milk samples. TPF procedure was optimized for extraction of MT from immature milk sample. The extraction of MT was performed from 1.0 ml of milk that contain 0.2 ml of Briton Robinson buffer (pH=2.5) and 0.3 ml of acetonitrile. For separation of acetonitrile from aqueous solution, the solution was placed in refrigerator at −40 °C. The MT was analyzed by gas chromatography. The results demonstrated that the amount of MT that transferred to milk is significantly different from other published reports. The immature milks of six women who were used MT (dose of 90 mg/day) in duration of 1, 2, 3, and 5 h after consumption were analyzed. Our data demonstrated that, before one hour and also 5 h after MT consumption the breastfeeding is safe and between 2-4 h after consumption dose not safe neither.


How to cite this article:
Jalilian N, Ahmadi F, Farhadi E. Two phase solvent extraction method for analysis of methadon in immature human milk, a breast feeding recommendation in early postpartum period.J Rep Pharma Sci 2018;7:9-19


How to cite this URL:
Jalilian N, Ahmadi F, Farhadi E. Two phase solvent extraction method for analysis of methadon in immature human milk, a breast feeding recommendation in early postpartum period. J Rep Pharma Sci [serial online] 2018 [cited 2018 May 22 ];7:9-19
Available from: http://www.jrpsjournal.com/article.asp?issn=2322-1232;year=2018;volume=7;issue=1;spage=9;epage=19;aulast=Jalilian;type=0