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Therapeutic effect of Ferulago angulata extract on reproductive parameters and serum testosterone levels in diabetic male rats
Glavizh Rostami Nassab, Somayeh Bohlouli, Ali Ghanbari
January-June 2018, 7(1):1-8
Diabetes is an important metabolic disease inducing different effects on body organs, especially reproductive system. Increased oxidative stress and antioxidant capacity changed in diabetes induce infertility and decrease the sperm parameters. This study was to evaluate the therapeutic effects of hydro alcoholic extract of Ferulago angulata on reproductive parameters in diabetic male rats. In this experimental study, we used 30 male Wistar rats (230- 250g) with an average age of 10 weeks. A total of 24 rats were made diabetes type I by 40 mg/kg streptozotosin. Animals were divided into 5 groups of control, diabetic, and diabetic+ Ferulago angulata extract (100, 200 and 400mg/kg). Sperm parameters, serum testosterone level, seminiferous tubules diameter, and germ line epithelium maturity were assayed at the end of study. Data were analyzed by one-way ANOVA test and P<0.05 was considered statistically significant. Our results showed serum testosterone level and sperm parameters, including count, viability, progressive motility, and normal morphology as well as seminiferous tubules diameter and germ line epithelium maturity of diabetic male rats increased at 200 and 400 mg/kg doses of Ferulago angulata extract (P<0.05). The hydroalcoholic extract of Ferulago angulata, an herbal plant with abundant antioxidants, improved the quality of sperm and reproductive parameters in diabetic male rats.
[ABSTRACT]   Full text not available  [PDF]
  670 530 -
Apoptosis cell death effect of linoleic acid from nigella sativa on human ovary cancer cells through mitochondrial intrinsic pathway
Yalda Shokoohinia, Gholamreza Bahrami, Fatemeh Taherabadi, Fataneh Jaffari, Leila Hosseinzadeh
January-June 2018, 7(1):20-26
In this study, we evaluated the cytotoxic potential of fractions (F1-F5) isolated from hexane extract of the seeds of N. sativa on human ovarian carcinoma cell line, A2780. F2 showed an outstanding potent cytotoxic effect against A2780 cells. Next, this fraction was purified to obtain six sub-fractions (SF1-SF6) and their cytotoxic effects were then evaluated. The obtained results showed that SF2 had strong cytotoxic effect against A2780 cell line. The effective sub-fraction (SF2) was determined to be linoleic acid (LA) according to spectroscopic analyses. In the next set of experiments, the apoptotic potentials of LA were investigated. Induction of apoptosis by LA was accompanied by an increase in activation of caspase-3, -9 and reduction in mitochondrial membrane potential (MMP) in A2780 cells. It can be concluded that LA, inhibited the growth of human ovarian carcinoma cells, A2780 and induced mitochondrial-related apoptosis.
[ABSTRACT]   Full text not available  [PDF]
  754 338 -
Two phase solvent extraction method for analysis of methadon in immature human milk, a breast feeding recommendation in early postpartum period
Nasrin Jalilian, Farhad Ahmadi, Elham Farhadi
January-June 2018, 7(1):9-19
In this work we introduced a new two phase freezing (TPF) method coupled with gas chromatography for the extraction, clean up and determination of methadone (MT) in human milk samples. TPF procedure was optimized for extraction of MT from immature milk sample. The extraction of MT was performed from 1.0 ml of milk that contain 0.2 ml of Briton Robinson buffer (pH=2.5) and 0.3 ml of acetonitrile. For separation of acetonitrile from aqueous solution, the solution was placed in refrigerator at −40 °C. The MT was analyzed by gas chromatography. The results demonstrated that the amount of MT that transferred to milk is significantly different from other published reports. The immature milks of six women who were used MT (dose of 90 mg/day) in duration of 1, 2, 3, and 5 h after consumption were analyzed. Our data demonstrated that, before one hour and also 5 h after MT consumption the breastfeeding is safe and between 2-4 h after consumption dose not safe neither.
[ABSTRACT]   Full text not available  [PDF]
  636 340 -
Synthesis of novel amide containing schiffs bases of 5-(4-chloro-phenyl)-furan-2-carboxaldehyde: Their In Vivo anti-inflammatory, antioxidant and antinociceptive activities with ulcerogenic risk evaluation
Saqlain Haider, Mohammad Sarwar Alam, Hinna Hamid, Sadiq Umar, Deepak Kumar, Syed Nazreen
January-June 2018, 7(1):44-63
A library of eighteen amide containing Schiffs bases has been synthesized and screened for their anti-inflammatory, antioxidant and antinociceptive activities. The compound 2 (COX-1 IC50 = 63.23 μM; COX-2 IC50 = 1.80 μM; SI = 35.12) exhibited potent selective COX-2 inhibition as compared to indomethacin (COX-1 IC50 = 3.60 μM; COX-2 IC50 = 7.50 μM; SI = 0.48). The compounds 2 and 7 reduced the COX-2 level to 7.5 ±0.35 nmole/min/ml and 6.8 ± 0.32nmole/min/ml respectively. The compounds 6 exhibited reduced the TNF-α level to 3.36 ± 0.18pg/ml. The compounds 2, 6, 7, 13 and 17 did not induce any gastric ulceration in comparison to the standard drug indomethacin.
[ABSTRACT]   Full text not available  [PDF]
  578 396 -
Gas chromatographic analysis of sodium valproate in plasma and urine after air assisted liquid-liquid microextraction
Maryam Abbaspour, Mir Ali Farajzadeh, Maryam Khoubnasabjafari, Sajad Haririan, Abolghasem Jouyban
January-June 2018, 7(1):27-43
Rapid, highly efficient, and reliable liquid–liquid microextraction (LLME) methods followed by gas chromatography-flame ionization detection were developed for the extraction, preconcentration, and determination of valproate in human plasma and urine samples. Proteins of plasma sample are precipitated by adding methanol and urine sample was diluted prior to performing the microextraction procedures. Fine organic solvent droplets were formed by repeated suction and injection of the mixture of sample solution and extraction solvent into a test tube with a glass syringe. After extraction, phase separation was performed by centrifuging and the enriched analytes in the sedimented organic phase were determined by the separation system. The main factors influencing the extraction efficiency including extraction solvent type and volume, salt addition, pH, and extraction times are investigated. Under the optimized conditions, the proposed method showed good precision (relative standard deviation less than 8%). Limits of detection and lower limits of quantification for valproate were obtained in the ranges of andμg mL-1, respectively. The linear ranges were 0.5–500 and 0.1–200 μg mL-1in plasma and urine, respectively (r2 ≥ 0.9995). The relative recoveries varied from 98–102 % and 93-100 %, respectively for plasma and urine samples. The mean relative standard deviations for intra-assay and inter-assay precisions were 3.4 % and 6.0 %, respectively. Preconcentration factors were in the range of 7-44. Good recoveries (55–86%) were obtained for the spiked samples. The proposed method was successfully used to analyze plasma and urine samples of epileptic receiving sodium valproate.
[ABSTRACT]   Full text not available  [PDF]
  514 307 -
Preparation and pharmaceutical evaluation of ferrous sulfate and ascorbic acid floating matrix tablet for prevention of anemia
Rahim Najafi Bahri, Lotfollah Saghaei, Taher Babaeimehr
July-December 2012, 1(2):72-80
For prolonging the time that drug remains in stomach, new methods used as floating drug delivery systems, that available in various forms such as floating tablets. These systems enhance drug absorption and decrease plasma concentration fluctuations. Iron deficiency and its inadequate absorption in diet are of community health problems. Common forms of iron available in the market have little bioavailability and due to greater excretion of drugs from the gastrointestinal tract has many complications such as constipation. Using floating systems to enhance drug absorption can reduce the dose required and drug side effects. In this study, preparation of floating tablets of ferrous sulfate plus ascorbic acid is considered since it has proven that vitamin C enhances iron absorption. Tablets were prepared with swollen polymers like HPMC K4M and carbopol934 by direct compression method. Sodium bicarbonate and citric acid was used to create the CO2 then drug properties such as buoyancy, release percentage and physical properties were tested on that. Tablet formulation No. 10, started to float in 27 seconds and floating state lasts 18 hours in environments like stomach. According to the great drug release and long floating state (12 h), tablet formulation 10 is recommended as a drug supplement to prevent anemia.
[ABSTRACT]   Full text not available  [PDF]
  630 129 -
Study of callus induction and cell culture to secondary metabolite production in Hyssopus officinalis L.
Gulsum Pakseresht, Danial Kahrizi, Mohsen Mansouri, Tayebeh Ghorbani, Nastaran Kazemi
July-December 2016, 5(2):104-111
The Hyssopus officinalis L. is an important medicinal plant. Antimicrobial and antifungal activities of the essential oil of hyssop have been reported. In this article the effects of explants types, plants growth regulators and elicitors on callus induction and cell culture conditions were studied. In this experiment it was found that there were non- significant differences among hypocotyl and leaf H. officinalis explants for callus induction and callus growth rate. There were significant differences among levels of growth regulators and interaction effect between growth regulators and expellant types for callus induction and callus growth rate. There were significant differences among plant growth regulators levels for callus induction and callus growth rate. Results showed that medium supplemented by N2B1 and N0.5B1 showed the highest callus induction and callus growth rate respectively. In this paper it was demonstrated the important role of plant growth regulators and explant types on callus induction in H. Officinalis as a medicinal plants. Also the most important secondary metabolites in H. officinalis were increased in cell culture presence of salicylic acid, citric acid and yeast extract elicitors.
[ABSTRACT]   Full text not available  [PDF]
  444 87 -
Antimicrobial potential of root, stalk and leaves extracts of Rheum Ribes
Reza Kazemi Darsanaki, Maryam Parsa Lisar
January-June 2014, 3(1):10-13
Plants, as sources of medicinal compounds have continued to play a dominant role in the maintenance of human health since ancient times. Rheum ribesis from Polygonaceae family which is endemic to Iran and a few neighboring countries. In this investigation, antimicrobial effects of root, stalk and leaves methanol extracts of R. ribesagainstStaphylococcusaureus, Escherichia coli, Klebsiellapneumoniae, Pseudomonas aeruginosa, Shigellaflexneri were studied, using well diffusion method. Methanol extracts obtained from root, stalk and leaves of R. ribes exhibited antimicrobial activity against test micro-organisms. R. ribes extracts were found to be more active against S. flexneri and K. pneumonia withinhibitory, 13.75 and 13.5 mm. The results suggested that extracts of R. ribes could be effectively used against diseases caused by selected human pathogens.
[ABSTRACT]   Full text not available  [PDF]
  467 52 -
Optimization of lipofectamine-2000/siRNALipoplexLoaded PLGANanoparticles for efficient EGFR gene silencing: An in Vitro study
Ali Fattahi, Behzad Shahbazi, Leila Hosseinzadeh, Ghobad Mohammadi
January-June 2018, 7(1):64-78
In this study, a novel small interfering RNA (siRNA) delivery system based on encapsulation of lipolexes was introduced. A Lipofectamine-2000–siRNA complex was encapsulated in particles of poly (D,L-lactic-co-glycolic acid; PLGA) by double micro-emulsion. Parameters such as surfactant concentration, the volume of the inner water phase and the outer water phase were evaluated to achieve high loading efficiency, small particle size and low polydispersity. The ratio of the internal to the external phase has a significant effect on the particle size and encapsulation efficiency. The various concentration of surfactant has a different effect on the particle size. In order to achieve optimum conditions for siRNA delivery, the luciferase siRNA was used as a reporter gene. The prepared formulations have a particle sizes in the range of 222 ± 5.2 nm to 900 ± 20 nm and loading efficiency in the range of 4% to 29%. lipoplex loaded PLGA particles (LPPs) had a zeta potential values ranging from −23±2.5 to −29±1.5 mV. S1 and S3 formulations showed greater efficiency compared to the lipoplexes. The gene silencing pattern of LPPs was different from lipoplex. The cytotoxicity of lipoplex loaded PLGA particles (LPPs) was lower than lipoplexes in H1299 cell line. LPPs showed better stability and higher level transfection in the presence of heparin than lipoplexes. The EGFR silencing of S1 formulation was greater than other formulation in A431 cell line. All together these properties suggest that lipoplex loaded PLGA particles have strong potential as a gene carrier for in vivo silencing angiogenesis and treatment of cancer.
[ABSTRACT]   Full text not available  [PDF]
  375 140 -
Formulation and evaluation of co-amoxiclav 228 and 312 mg dispersible tablets
Abolfazl Aslani, Azam Fathi
January-June 2016, 5(1):1-11
Dispersible tablet is a new form of tablet which any dose of it is dispersed in a small amount of water to create a uniform suspension. The advantage of this formulation is its better and faster effect, no need to swallow, more stability compared to suspension, better taste and greater acceptance by the patients especially kids and elders. This study aimed to design and formulize dispersible tablets including Co- Amoxiclav 228 and 312 mg for enhancement of stability and easier usage and consumption. Co amoxiclav dispersible pill was made by dry granulation method using superdisintegrants ingredients such as crospovidone, croscarmellose sodium and sodium starch glycolate and effervescent materials such as citric acid and sodium bicarbonate. The mixed powder was tested in terms of compressibility, particle size distribution and powders flowability. Some tests were performed for determination of assay, content uniformity, hardness and friability of tablet, weight variation, wetting time, water absorption ratio and disintegration of tablets. Prepared granules had good flowability, compressibility and the hardness and friaibility of tablets were in an acceptable range in most formulations. Formulations made by effervescent bases E3 and E4 had disintegration time of 25 and 35 seconds and S1, S4 and S5 formulations made from superdisintegrants materials showed the disintegration time of 260, 262 and 275 seconds. E4 formulation containing amoxicillin trihydrate, potassium clavulanate, citric acid, sodium bicarbonate, manitol, aspartame and PEG 6000 had 25 sec disintegration time and 40 N hardness.
[ABSTRACT]   Full text not available  [PDF]
  426 74 -
Formulation and evaluation of orally disintegrating tablets of captopril using natural super disintegrants
Mohammad Ali Shahtalebi, Majid Tabbakhian, Navid Sarbolookzadeh Harandi
January-June 2014, 3(1):54-64
Difficulty in swallowing (dysphagia) is common among all age groups, especially in elderly and children. Orally Disintegrating tablets constitute an innovative dosage form that overcomes the problems of swallowing and provides a quicksetof action. The purpose of this study was to formulate and evaluate an orally Disintegrating Tablet (ODT) containing captopril while using croscarmellose sodium, crospovidone and two natural superdisintegrants: karaya gum and natural agar. For the preliminary study 12 batches were prepared. A 32 full factorial design was applied to optimize the formulation and 9 batches were prepared and evaluated. From the preliminary study it was found that ODTs containing karaya gum showed a better disintegration time and hence it was considered for further studies. According to the Results of optimized batches, the best concentration of superdisintegrant and binder were obtained. Karaya gum in the concentration of 9% w/w with Avicel PH 102 in25% w/w gave rapid disintegration in 25sec and showed 100% drug release within 5 minuteso it was concluded that orally disintegrating tablets of captopril can be successfully formulated using karaya gum.
[ABSTRACT]   Full text not available  [PDF]
  354 83 -
Design, formulation and evaluation of its physiochemical properties of acetaminophen, ibuprofen and caffeine as effervescent tablet
Abolfazl Aslani, Ali Daliri
July-December 2016, 5(2):122-134
The aim of this study was to design, formulate and evaluate the physicochemical properties of acetaminophen, ibuprofen and caffeine as effervescent tablets, since, they can overcome the problems with drug swallowing for the pediatric, elderly and bed-ridden patients. Effervescent tablets were prepared in a dosage of 325 mg acetaminophen, 200 mg ibuprofen and 40 mg caffeine by fusion and direct compression methods. Pre-compression characteristics of the mixed powders and granules, such as angle of repose, compressibility index, mean particle size and Hausner's ratio were evaluated. Then, they were evaluated for post-compression properties including weight variation, hardness, friability, carbon dioxide content, effervescence time, pH, content uniformity, assay and water content. Panel taste was performed using 30 volunteers. After performing the required procedures, citric acid and sodium bicarbonate were selected as effervescent materials. It was resulted that the fusion method was exhibited more flowability than direct compression and the G5 formulation was selected as the optimized formulation. It is significant that fusion method resulted in better tablets compared to direct compression method.
[ABSTRACT]   Full text not available  [PDF]
  337 84 -
Neuroprotective effects of medicinal plants and their constituents on different induced neurotoxicity methods: A review
Reza Mohebbati, Mohammad Reza Khazdair, Mahdiyeh Hedayati
January-June 2017, 6(1):34-50
In the traditional medicine, numerous plants have been used to treat cognitive disorders. Natural products play an essential role in prevention and therapy of various neurodegenerative diseases, and neuronal dysfunctions. Different studies suggest that natural products, such as polyphenolic and alkaloids compounds that isolated from plants potentially delayed the neurodegeneration and also improve memory and cognitive function. Ethno pharmacological studies have provided information to identify potential new drugs from plant sources. Recently many drugs which available in medicine were originally isolated from plants or their constituents including, anticholinesterase (Anti-ChE) alkaloids isolated from plants have been investigated for their potential in the treatment of Alzheimer's disease (AD). Anti-inflammatory and antioxidant activities of plants and isolated components form plants were used in improvement neuro-inflammation, anxiety, convulsion and etc. Medicinal plants have beneficial properties due to presence of various complex chemical substances for treatment of toxicity in the nervous system. This review article evaluated the some of the medicinal plants and their active constituents that have been used in different methods induced neurotoxicity.
[ABSTRACT]   Full text not available  [PDF]
  334 82 -
Formulation direct compression tablet of probiotic as vehicle for oral delivery
Shahla Mirzaeei, Shiva Tagheh
January-June 2017, 6(1):84-95
There has been an explosion of probiotic health-based products. Many reports indicated that there is poor survival of probiotic bacteria in these products. For oral delivery of probiotic, providing living cells with a physical barrier against adverse environmental conditions is considerable interest. The main goal of the study was to develop tablets made out of functional polymers in order to protect probiotic bacteria from gastric acidity, thus providing an easily manufacturing scale-up dosage form to deliver probiotics to the human intestinal. Tablets were produced by direct compression using Microcrystalline Cellulose) Avicel® (as main exipient. To optimize the formulation, using survival rate after both compressing and exposed pH: 2 acid medium were evaluated. Storage stability of lactobacilluse acidophiluse tablets was also performed by evaluation of viable cells throughout 3 months at 4 °C. The highest viability was found in formulation 7 and 8 with %90.37 and 90.27% after compression pressure. Increasing amount of Avicel® in the tablet increase bacterial viability against pressure. 72.51% Survival was showed is related to formulation 3 after exposure acid. It can be concluded that The best protective qualities against artificial gastric juice were observed when tablets were prepared from compaction mixtures of lactic acid bacteria (LAB), sodium alginate, and Xanthan. A decrease of approximately one logarithmic cycle was observed after 3 month storage for formula 3 while untreated cell decrease 3.97 log. This preparation method and tablet formulation can be employed for intestinal delivery to ensure maximum viable cell release at intestine or colon and this product remains stable until the time of consumption.
[ABSTRACT]   Full text not available  [PDF]
  341 74 -
Pharmacognostic standardization and chromatographic fingerprint analysis on triterpenoidal constituents of the medicinally important plant Artocarpus heterophyllus by high-performance thin layer chromatography technique
Gunja Srivastava, Manjul Pratap Singh
January-June 2019, 8(1):1-12
Background: Artocarpus heterophyllus commonly known as Kathal in Hindi and Jackfruit in English has a wide horizon of medicinal possessions. The plant is found in India and in its tropical regions. Objective: Although the plant and its extracts are renowned for its ethnic medicinal values diversely in India, yet organized data somewhere lack in reverse pharmacognostical approach of this plant that shows that plant have not been completely explored for its therapeutic potency. Materials and Methods: In the present study, the folklore potential of this plant has been explored by generating down its pharmacognostical standards along with measurement of its active therapeutic constituent ursolic acid and lupeol via. High-performance thin layer chromatography (HPTLC), evidence from organized data search says that ursolic acid and lupeol is ubiquitous to A. heterophyllus. The plant was also subjected to spectroscopic-based estimation of tannins (gallic acid and tannic acid) and flavonoids (quercetin and rutin). Antimicrobial testing was also performed. Results: Microscopic features revealed the presence of anomocytic type of stomata, collateral open type vascular bundle in which fascicular cambium is present, calcium oxalate crystal and covering trichomes were key features in leaves. Methanolic extract of leaves of the plant was subjected to HPTLC. HPTLC studies revealed that both ursolic acid and lupeol are present in appreciable amount. Plant showed good antibacterial activity which may be due to the high amount of tannins as the tannins has the ability to disintegrate the bacterial cell wall. Conclusion: The data generated could be significantly used as a reference for the authentication and quality control of A. heterophyllus.
  300 94 -
Analytical and physicochemical characterization of cefquinome using capillary electrophoresis
Leila Behbood, Yahya Mrestani, Ralf Warrass, Reinhard Neubert
July-December 2017, 6(2):96-104
The Capillary Electrophoresis (CE) is a very useful technique to determine and to quantify pharmaceutical materials. In this work, a capillary electrophoresis method has been developed for the determination of Cefquinome sulfate in aqueous solution and in biological media (urine, milk and plasma). The ion mobility was measured at the various pH values. The detection limit and the relative standard deviation of migration times of the peak area were determined. The influence of the pH on the separation of Cefquinome sulfate in plasma and in urine was investigated. The SDS micellar elecrokinetic chromatography (SDS-MEKC) was used for the determination of Cefquinome sulfate in water, plasma and urine. The solubility and stability of Cefquinome sulfate were considered at the different pH values. The best stability was found at pH 7.4. Also the hydrophilic/lipophilic properties of Cefquinome sulfate (partition coefficient and permeation, in vitro) were investigated. The hydrophilic properties of cefquinome affected the results; the partition coefficient was 0.01 at pH 7.4. In addition, the permeation coefficient was 1.65 at pH 7.4. Acid the dissociation constant of cefquinome was calculated using CZE. The low amount of sample required and the relatively short analyze time were the main advantages of this method.
[ABSTRACT]   Full text not available  [PDF]
  297 72 -
Design and evaluation of soluble ocular insert for controlled release of chloramphenicol
Shahla Mirzaeei, Moslem Alizadeh
July-December 2017, 6(2):123-133
Soluble ocular inserts of chloramphenicol were prepared with the aim of achieving once a day administration. Drug reservoir was prepared using hydrophilic polymer and rate-controlling hydrophobic polymer; Eudragit L100, Eudragit S100, Eudragit RL100. All the formulations indicated no interaction between drug and polymer in FTIR studies. The Inserts were evaluated for the several parameters, viscosity, drug–polymer interaction, in vitro drug release, sterility testing. They were also evaluated for % moisture loss, % moisture uptake,thickness, and tensile strength. Ophthalmic inserts provide that prolonged and sustained drug release. Ocular inserts prepared were smooth and passed all the evaluation tests performed. Mechanical properties and in vitro drug release were dependent on film composition. The release profile of all the formulations showed a steady, controlled drug release. Ocular inserts formulated also passed the test for sterility. In vitro studies demonstrated that P5 insert can ensure a sustained drug release on the ocular surface for a prolonged over 20 hours' time period. Also, reduction in frequency of administration, may improve the patient compliance.
[ABSTRACT]   Full text not available  [PDF]
  279 72 -
A spectrophotometric method for the determination of aldehyde oxidase activity using 3-methyl-2-benzothiazolinone hydrazine: A preliminary study
Maryam Siah, Mohammad Hosein Farzaei, Mohammad Reza Ashrafi-Kooshk, Hadi Adibi, Reza Khodarahmi
January-June 2017, 6(1):23-33
Aldehyde oxidase, a molybdenum cofactor-containing cytosolic enzyme, is extensively distributed throughout the animal kingdom. The enzyme is mainly active in liver and other tissues of mammalian species and involved in the metabolism of wide range of aldehydes and nitrogen-containing molecules. A continuous spectrophotometric method for the quantitative determination of aldehyde oxidase, AO, enzyme activity is described in this article. This method is based on the coupling reaction between 3-methyl-2-benzothiazolinone hydrazone (MBTH) and the o-quinone. Dopamine as substrate for AO, is converted/oxidized to o-quinone. The latter react with MBTH to produce intensely colored products that absorb light maximally in the visible region. Then, AO activity has been kinetically characterized; the Km (Michaelis–Menten constant) and Vmax (maximum initial velocity) values for the oxidation of dopamine by AO were evaluated. The existence of MBTH in the reaction medium and production of stable color as well as the high ϵ values at 510 nm of MBTH-Q adduct make this direct technique more sensitive than other continuous methods for AO assay. The optimized MBTH reaction may be useful for biological staining of AO activity isolated from various biological sources in electrophoresis gels.
[ABSTRACT]   Full text not available  [PDF]
  276 74 -
Hepatoprotective effects of combination hydroalcoholic extracts of Nigella Sativa and Curcuma Longa on adriamycin-induced oxidative stress in rat
Reza Mohebbati, Mohammad Reza Khazdair, Sareh Karimi, Abbasali Abbasnezhad
July-December 2017, 6(2):105-114
The ample of studies show that Adriamycin caused hepatotoxicity in rat. The present study investigated the effects of Nigella Sativa (N. Sativa) and combination with Curcuma longa (C. longa) extract on Adriamycin-induced hepatotoxicity in rat. Rats were divided into eight experimental groups: Control (CO), Adriamycin (ADR), Vitamin C (Vit C), Adriamycin with Vitamin C (ADR+VitC), N. Sativa with and without Adriamycin (NS +ADR, NS -ADR), Combination extract of C. longa and N. Sativa with and without Adriamycin (NS+CL+ADR, NS+CL-ADR). Malondialdehyde (MDA) and thiol levels and also the activities of catalase (CAT) in liver tissue were evaluated. MDA level in the liver tissue in ADR was increased compared to CO group but in NS+ADR group, ADR+VitC and NS –ADR groups decreased compared to ADR group. Thiol levels in ADR and ADR+VitC groups were decreased compared to CO group. Thiol levels in treatment groups were increased compared to ADR group. The activities of CAT in liver tissue of ADR group were lower than CO group, and increased in treatment groups comparison with ADR group. The results showed that chronic administration of N. sativa hydroalcoholic extract in Adriamycin-induced hepatotoxicity rats could decrease the oxidative stress injuries in liver tissue.
[ABSTRACT]   Full text not available  [PDF]
  276 69 -
Investigation on the effects of Bactenecin on POPC membrane in atomistic details using molecular dynamics simulation
Seyran Saeidi, Elham Esmaeili, Mohabbat Ansari, Sajad Moradi, Mohsen Shahlaei
January-June 2019, 8(1):13-17
Background: Traditional antimicrobial agents are losing their efficiency as microbial resistance increases. Thus, developing antimicrobial peptides (AMPs) can assist as an alternative approach. For AMPs, the hypothesis mode of action is involved in pore formation within the lipid membrane, thereby leading to cell death. In this study, interaction between Bactenecin and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylcholine (POPC) was studied. Methods: For this purpose, two systems, Bactenecin in water and Bactenecin in POPC were treated by 50 ns of molecular dynamic simulation and data were compared with those of free POPC. Results: The results suggest that the interaction between Bactenecin and bilayer membrane cause some disorder and more instability along with little compactness of bilayer. The hydrogen bond between peptide and heads of lipid components may is main reason of membrane compactness. The results can provide some information on how to Bactenecin or other such peptides affect bio-membranes.
  274 66 -
Preparation and characterization of camptothecin grafted chitosan oligosaccharide nanomicelles
Babak Tajani, Reza Tahvilian, Saeideh Khazaei, Komail Sadr Javadi, Ali Fattahi
January-June 2015, 4(1):1-11
Water soluble chitosan oligosaccharide (CHO) was hydrophobically modified with camptothecin (CPT) in different molar ratios of CPT, and characterized by FT-IR and 1H-NMR spectroscopy. Nanomicelles prepared by these copolymers were analyzed for size, zeta, and length of time they remain stable. The critical micelle concentration (CMC) and dissociation of micelles at physiological pH and acidic pH were also investigated. FT-IR and 1H-NMR spectroscopy approved chemical modification of CHO. Fluorometry study showed that the CMC of grafted copolymer decreased from 27.2×10 to 6.1×10 g/L with an increasing feeding ratio from 2.5 to 7.5. Particle size of CPT-CHO-2.5 and CPT-CHO-7.5 was 539 ± 152.15 and 197.8±66 nm, respectively. The dissociation study showed that the micelles are stable, and the disassembly was not observed in water at 37°C neither at pH of 7.4 nor at pH of 5.5. These phenomena make CPT-CHO micelles as stable polymeric micelles for drug delivery.
[ABSTRACT]   Full text not available  [PDF]
  271 68 -
Biomass of Aspergillus Niger: Uses and applications
Mohsen Shahlaei, Alireza Pourhossein
January-June 2013, 2(1):83-89
Fungal biomass of Aspergillus Niger, is a byproduct of citric acid fermentation that has proven to be a valuable biomaterial that can be both beneficial and practical. From an environmental point of view, it can be a useful bioadsorbent to detoxify and decolorize the wastewater samples. In industrial and pharmaceutical applications, it can also be used as an inexpensive and desirable source for commercial production of some expensive and useful biopolymers such as chitin, chitosan and food supplements such as chitin-glucan and glucosamine.
[ABSTRACT]   Full text not available  [PDF]
  292 46 -
Mucoadhesive electrospun nanofibers of chitosan/gelatin containing vancomycin as a delivery system
Leila Behbood, Pouran Moradipour, Fayegh Moradi, Elham Arkan
July-December 2017, 6(2):150-160
Mucoadhesive oral delivery systems have been of interest to researchers because of improving bioavailability and patient compliance by increasing concentration gradient. This study aimed to investigate local delivery of vancomycin using against gram-positive bacteria. Vancomycin is not absorbed from the gastrointestinal tract, so it will be given by injection. Also, slow infusion of vancomycin in blood is so vital to reduce its severe side effects and is excreted through the urine. Controlled dose of vancomycin can reduce drug resistance. Here in, a blend of chitosan and gelatin loaded with vancomycin was electrospun. The obtained nanofibers were characterized by FTIR, UV and SEM. The swelling and stability of the nanofibers were evaluated. In vitro release of the vancomycin was measured over a 72-hours period by total immersion method. The average diameter of drug-loaded nanofiber was 384 nm. The release behavior of the optimum fibrous mat conform Higuchi kinetic model.
[ABSTRACT]   Full text not available  [PDF]
  268 70 -
A linear principal component regression and nonlinear neural network model for determination of indomethacin in plasma samples using UV-Vis spectroscopy and comparison with HPLC
Gholamreza Bahrami, Hamid Nabiyar, Komail Sadr Javadi, Mohsen Shahlaei
January-June 2015, 4(1):82-100
A sensitive and selective method using combination of two chemometrics methods, principal component Analysis (PCA) and artificial neural network (ANN), and UV-Visible spectroscopy has been developed for the determination of Indomethacin (IDM) in plasma samples. Initially the absorbance spectra were processed using PCA to noise reduction and data compression. The scores of these PCs were used as the inputs of ANN. The ANN trained by the back-propagation learning was employed to model the complex non-linear relationship between the PCs extracted from UV-Visible spectra of IDM and the absorbance values. Nonlinear method (PC-ANN) was better than the PCR method considerably in the goodness of fit and predictivity parameters and other criteria for evaluation of the proposed model. Optimal ANN model were as follows: Number of input PCs: 2, number of neurons in hidden layer: 3. The linear calibration range was 1×10-7 to 2.4×10-6 M, the detection limit were 0.21 × 10-7 M., The results have been compared with those obtained by the HPLC method.
[ABSTRACT]   Full text not available  [PDF]
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Detection of corticosteroid compounds and phosphodiesterase inhibitors (PDH-5) as counterfeit in herbal products available in Iranian market by HPLC method
Ronak Jalili, Shahram Miraghaei, Bahareh Mohamadi, Atefeh Babaei, Gholamreza Bahrami
January-June 2015, 4(1):75-81
Weight-gain and potency enhancing drugs are the most popular herbal products in Iran which are easily available via the internet and through illegal markets. The content of some of these popularly purchased products were investigated for the presence of illegal substance like phosphodiesterase inhibitors (PDH-5) and corticosteroids by HPLC method. For this study, ten samples of both kind of herbal medicine were obtained from Iranian markets, then chromatographic analysis of corticosteroids was achieved isocratically on a C18 column (C18, 5μm , 150 mm × 4.6 mm) by utilizing a mobile phase of methanol/water (55:45, v/v, pH 7.0) at a flow rate of 1.5 mL/min with UV detection at 250 nm, while analysis of phosphodiesterase inhibitors followed by a C18 column (C18, 5μm, 150 mm × 4.6 mm) using a mobile phase of methanol/water (65:35 v/v, diethyl amine(100μl/l, pH 3.5) at a flow rate of 1.5 mL/min. UV detector operated at 290 nm. After quantitative analysis, different contents of sildenafil and tadalafil in 3 cases of enhancing herbal remedies and dexamethazone in 3cases of weight gaining herbal medicine have been identified.
[ABSTRACT]   Full text not available  [PDF]
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