• Users Online: 456
  • Home
  • Print this page
  • Email this page
Home About us Editorial board Ahead of print Current issue Search Archives Submit article Instructions Subscribe Contacts Login 
Year : 2020  |  Volume : 9  |  Issue : 1  |  Page : 41-45

Improvement of antiproliferative activity of recombinant truncated form of Pseudomonas aeruginosa exotoxin (PE38) by vitamin E in MCF-7 cells

1 Department of Genetics and Biotechnology, School of Biological Science, Varamin-Pishva Branch, Islamic Azad University, Varamin, Iran
2 Research and Development Laboratory, Javid Biotechnology Institute, Tehran, Iran
3 Department of Pharmaceutical Biotechnology, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Correspondence Address:
Dr. Atieh Hashemi
Department of Pharmaceutical Biotechnology, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran.
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/jrptps.JRPTPS_114_19

Rights and Permissions

Background: In addition to beneficial roles of vitamin E in many metabolic processes and its antitumor activities, vitamin E derivatives have extensively been considered as permeability enhancers. Using these enhancers, permeability of a wide spectrum of drugs was reported to be significantly increased. PE38, a toxic substance with a potential application in cancer therapy, is a truncated form of Pseudomonas exotoxin A (PE), which lacks Ia and a portion of domain Ib. Objective: Here, the antiproliferative potential of PE38 and alpha-tocopherol (αT) form of vitamin E were assessed in MCF-7 cells. The role of vitamin E in PE38 cytotoxicity level was also evaluated. Materials and Methods: The antiproliferative potential of PE38, vitamin E, and a combination of them were colorimetrically evaluated in a cellular breast cancer model (MCF-7), using the MTT assay. P values of <0.05 were considered significant. Results: Compared to control cells, the PE38 inhibited the proliferation of MCF-7 cells (80% ± 1.37% cell viability) only at the highest concentration used (500 μg/mL) (P < 0.05). MTT assay also showed that 0.1, 1, and 10 mg/mL of vitamin E could significantly (P < 0.001) decrease the cell viability of MCF-7 cells to 57% ± 1.37%, 26.8% ± 1.37%, and 14.7% ± 1.37% at 24 h, respectively. Moreover, the coadministration of vitamin E (0.1 mg/mL) with 31.25, 62.5, 125, 250, and 500 μg/mL concentrations of PE38 decreases in cell viability from 100% in control cells to 35.61% ± 4.29%, 37.8% ± 6.45%, 36.42% ± 5.79%, 32.33% ± 4.62%, and 29.97% ± 5.07% at 24 h, respectively (P < 0.001). Conclusion: The results of this study suggest that vitamin E can enhance the antiproliferative activity of PE38 toward MCF-7 cells.

Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)

 Article Access Statistics
    PDF Downloaded132    
    Comments [Add]    

Recommend this journal