Journal of Reports in Pharmaceutical Sciences

ORIGINAL ARTICLES
Year
: 2020  |  Volume : 9  |  Issue : 2  |  Page : 183--188

Apoptotic effects of ginger extract (Zingiber officinale) on esophageal cancer cells ESO26: An in vitro study


Abbas Abbasi1, Ali Azizi2, Seidmostafa Nachvak1, Elaheh Alizadeh1, Rezvan Abbsavaran1, Elham Mirtaheri1, Mehdi Moradinazar3, Mehrali Rahimi4 
1 Faculty of Nutrition and Food Industry, Pharmaceutical Science Research Center, Kermanshah, Iran
2 Department of Community Medicine, Faculty of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran
3 Assistant Professor in Epidemiology, Behavioral Disease Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran
4 Diabetes Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran

Correspondence Address:
Dr. Mehrali Rahimi
Department of Internal Medicine, Kermanshah University of Medical Science, Kermanshah.
Iran

Aim: Ginger is a natural dietary rhizome with antioxidant, anti-inflammatory, and anticarcinogenic properties. It has many medical beneficial properties such as anti-proliferation and antiapoptotic effects on cancerous esophageal cells. Materials and Methods: Esophageal cancer cells ESO26 were cultured in the presence and absence of ginger extract at various concentrations for 12, 18, and 24h. Then, the viability was determined by 3-(4,5-dimethylthiazol)-2,5-diphenyl tetrazolium bromide (MTT) assay. Western blot analysis of caspase-3 was performed to detect apoptosis. p21, Bax, and Bcl-2 gene expression was measured using quantitative polymerase chain reaction (PCR). Data were analyzed using one-way analysis of variance (ANOVA) followed by Tukey test. Results: The ginger extract increased the cleavage of caspase-3 in cells (P < 0.05). Results of real-time PCR have shown that ginger decreased the expression of Bcl-2 and increased Bax and p21 gene expression (P < 0.05). Conclusions: Results showed that the process of cell proliferation has been stopped. Also, this study indicated that ginger might exert a chemopreventive effect on esophageal cancer through the suppression of proliferation and the growth of tumor cells as well as the induction of apoptosis.


How to cite this article:
Abbasi A, Azizi A, Nachvak S, Alizadeh E, Abbsavaran R, Mirtaheri E, Moradinazar M, Rahimi M. Apoptotic effects of ginger extract (Zingiber officinale) on esophageal cancer cells ESO26: An in vitro study.J Rep Pharma Sci 2020;9:183-188


How to cite this URL:
Abbasi A, Azizi A, Nachvak S, Alizadeh E, Abbsavaran R, Mirtaheri E, Moradinazar M, Rahimi M. Apoptotic effects of ginger extract (Zingiber officinale) on esophageal cancer cells ESO26: An in vitro study. J Rep Pharma Sci [serial online] 2020 [cited 2021 Apr 11 ];9:183-188
Available from: https://www.jrpsjournal.com/article.asp?issn=2322-1232;year=2020;volume=9;issue=2;spage=183;epage=188;aulast=Abbasi;type=0