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  Indian J Med Microbiol
 

Figure 1: A. Effect of quercitrin on PE cell models toward (I) Live cells, (II) Early apoptosis (Apoptosis), (III) Late apoptosis (Death cells), and (IV) Necrotic cells. Treatments: (A) negative control (untreated cells), (B) vehicle control (cells+DMSO 1%), (C) positive control (hypoxia-induced cells), (D) positive control+quercitrin 25 µg/mL, and (E) positive control+quercitrin 6.25 µg/mL. *Data are presented as mean ± standard deviation. Single star (*) marks significant difference compared with the negative control group, and single hashtag (#) marks significant difference compared with the positive control (P < 0.05, Tukey’s HSD test). B. Representation of dot plots on hypoxia-induced Ea.Hy926 cells that are treated with quercitrin in various concentrations toward apoptosis percentage using flow cytometry. (I) Negative control (untreated cells): live cells: 99.80%, necrotic: 20.0%, early apoptosis: 0.0%, late apoptosis: 0.0%. (II) Vehicle control (cells + DMSO 1%): live cells: 96.36%, necrotic: 0.88%, early apoptosis: 1.77%, late apoptosis: 1.19%. (III) Positive control (hypoxia-induced cells): live cells: 84.06%, necrotic: 1.27%, early apoptosis: 1.91%, late apoptosis: 12.76%. (IV) Positive control+quercitrin 25 µg/mL: live cells: 95.29%, necrotic: 1.42%, early apoptosis: 1.90%, late apoptosis: 1.39%. (V) Positive control+quercitrin 6.25 µg/mL: live cells: 95.97%, necrotic: 1.36%, early apoptosis: 1.61%, late apoptosis: 1.06%

Figure 1: A. Effect of quercitrin on PE cell models toward (I) Live cells, (II) Early apoptosis (Apoptosis), (III) Late apoptosis (Death cells), and (IV) Necrotic cells. Treatments: (A) negative control (untreated cells), (B) vehicle control (cells+DMSO 1%), (C) positive control (hypoxia-induced cells), (D) positive control+quercitrin 25 µg/mL, and (E) positive control+quercitrin 6.25 µg/mL. *Data are presented as mean ± standard deviation. Single star (*) marks significant difference compared with the negative control group, and single hashtag (#) marks significant difference compared with the positive control (<i>P</i> < 0.05, Tukey’s HSD test). B. Representation of dot plots on hypoxia-induced Ea.Hy926 cells that are treated with quercitrin in various concentrations toward apoptosis percentage using flow cytometry. (I) Negative control (untreated cells): live cells: 99.80%, necrotic: 20.0%, early apoptosis: 0.0%, late apoptosis: 0.0%. (II) Vehicle control (cells + DMSO 1%): live cells: 96.36%, necrotic: 0.88%, early apoptosis: 1.77%, late apoptosis: 1.19%. (III) Positive control (hypoxia-induced cells): live cells: 84.06%, necrotic: 1.27%, early apoptosis: 1.91%, late apoptosis: 12.76%. (IV) Positive control+quercitrin 25 µg/mL: live cells: 95.29%, necrotic: 1.42%, early apoptosis: 1.90%, late apoptosis: 1.39%. (V) Positive control+quercitrin 6.25 µg/mL: live cells: 95.97%, necrotic: 1.36%, early apoptosis: 1.61%, late apoptosis: 1.06%